Ricci-Júnior Eduardo, Marchetti Juliana M
Faculty of Pharmaceutical Sciences, Laboratory of Pharmaceutical Technology, University of São Paulo, Ribeirão Preto, São Paulo, Brazil.
J Microencapsul. 2006 Aug;23(5):523-38. doi: 10.1080/02652040600775525.
Nanoparticles containing Zinc (II) Phthalocyanine (ZnPc) were prepared by a spontaneous emulsification diffusion method utilizing poly-(D,L lactic-co-glycolic acid) (PLGA), characterized and available in cellular culture. The process yield and encapsulation efficiency were 60% and 80%, respectively. The nanoparticles have a mean diameter of 200 nm, a narrow size distribution with polydispersive index of 0.15, smooth surface and spherical shape. ZnPc loaded nanoparticles maintain their photophysical behaviour after the encapsulation process. Photosensitizer released from nanoparticles was sustained with a burst effect of 10% for 3 days. The photocytotoxicity was evaluated on P388-D1 cells. They were incubated with ZnPc loaded Np by 6 h and exposed to light (675 nm) for 120 s, and light dose of 30 J cm-2. After 24 h of incubation, the cellular viability was determined, obtaining 60% of cellular death. All the physical-chemical and photobiological measurements performed allowed one conclude that ZnPc loaded PLGA nanoparticles are a promising drug delivery system for PDT.
采用自发乳化扩散法，利用聚（D，L - 乳酸 - 乙醇酸共聚物）（PLGA）制备了含锌（II）酞菁（ZnPc）的纳米颗粒，并对其进行了表征，且可用于细胞培养。该过程的产率和包封率分别为60%和80%。纳米颗粒的平均直径为200 nm，粒径分布窄，多分散指数为0.15，表面光滑且呈球形。负载ZnPc的纳米颗粒在包封过程后保持其光物理行为。从纳米颗粒中释放的光敏剂持续释放，3天内有10%的突发效应。在P388 - D1细胞上评估了光细胞毒性。将它们与负载ZnPc的纳米颗粒孵育6小时，然后暴露于675 nm的光下120秒，光剂量为30 J/cm²。孵育24小时后，测定细胞活力，细胞死亡率为60%。所进行的所有物理化学和光生物学测量表明，负载ZnPc的PLGA纳米颗粒是一种有前景的光动力疗法药物递送系统。
