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Reduction of background labeling in colloidal gold-enzyme reactions.

作者信息

Squarzoni S, Zini N, Marinelli F, Maraldi N M

机构信息

Istituto di Citomorfologia Normale Patologica CNR, Bologna, Italy.

出版信息

Histochemistry. 1990;94(3):297-301. doi: 10.1007/BF00266631.

DOI:10.1007/BF00266631
PMID:1698189
Abstract

Most methods which are used to prevent a specific colloidal gold (c.g.) binding in cytochemical reactions are based on the addition of macromolecules to the incubation medium. A new method based on the effects of glow discharge (GD) plasma is reported.

摘要

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本文引用的文献

1
Ultrastructural comparison of ion beam and radiofrequency plasma etching effects on biological tissue sections.离子束与射频等离子体蚀刻对生物组织切片影响的超微结构比较
J Microsc. 1984 Apr;134(Pt 1):101-12. doi: 10.1111/j.1365-2818.1984.tb00508.x.
2
Ultrastructural localization of nuclei acids by the use of enzyme-gold complexes.利用酶-金复合物对核酸进行超微结构定位。
J Histochem Cytochem. 1981 Apr;29(4):531-41. doi: 10.1177/29.4.6265546.
3
A procedure for the isolation of enzymically active rat-liver nuclei.一种分离具有酶活性的大鼠肝细胞核的方法。
Biochem J. 1964 Aug;92(2):313-7. doi: 10.1042/bj0920313.
4
Non-specific binding of protein-stabilized gold sols as a source of error in immunocytochemistry.蛋白质稳定的金溶胶的非特异性结合作为免疫细胞化学中误差的一个来源。
Eur J Cell Biol. 1986 Aug;41(2):326-38.
5
Phospholipase C digestion induces the removal of nuclear RNA: a cytochemical quantitative study.
Histochem J. 1989 Aug;21(8):491-500. doi: 10.1007/BF01845799.
6
Cytochemical localization of DNA loop attachment sites to the nuclear lamina and to the inner nuclear matrix.DNA环附着位点在核纤层和内核基质上的细胞化学定位。
Histochemistry. 1989;91(3):199-204. doi: 10.1007/BF00490133.
7
Prevention of non-specific interactions of gold-labeled reagents on tissue sections.
Histochemistry. 1989;92(1):47-56. doi: 10.1007/BF00495015.
8
Perinuclear dense bodies: characterization as DNA-containing structures using enzyme-linked gold granules.核周致密小体：利用酶联金颗粒鉴定为含DNA结构
J Cell Sci. 1986 Feb;80:1-11. doi: 10.1242/jcs.80.1.1.
9
Ultrastructure of cel organelles by scanning electron microscopy of thick sections surface-etched by an oxygen plasma.
J Microsc. 1979 Jul;116(2):255-64. doi: 10.1111/j.1365-2818.1979.tb00206.x.
10
Adsorption of horseradish peroxidase, ovomucoid and anti-immunoglobulin to colloidal gold for the indirect detection of concanavalin A, wheat germ agglutinin and goat anti-human immunoglobulin G on cell surfaces at the electron microscopic level: a new method, theory and application.辣根过氧化物酶、卵类粘蛋白和抗免疫球蛋白吸附于胶体金上用于在电子显微镜水平间接检测细胞表面的伴刀豆球蛋白A、麦胚凝集素和山羊抗人免疫球蛋白G：一种新方法、理论及应用
J Histochem Cytochem. 1977 Nov;25(11):1187-200. doi: 10.1177/25.11.21217.