Mistchenko Alicia Susana, Viegas Mariana, Latta Maria Paula Della, Barrero Paola Roxana
Comisión de Investigaciones Científicas de la Provincia de Buenos Aires, Virology Laboratory, Dr. Ricardo Gutiérrez Children's Hospital, Buenos Aires, Argentina.
J Clin Virol. 2006 Dec;37(4):293-9. doi: 10.1016/j.jcv.2006.08.009. Epub 2006 Sep 18.
Human enteroviruses are one of the major causes of central nervous system (CNS) infections in pediatrics.
We have studied 1242 children under 15 years old with suspicion of CNS infection from January 1998 to December 2003. CSF was obtained and molecular typing of human enterovirus B serotypes was performed by RT-PCR and sequencing of the N-terminal part of VP1 gene.
According to the clinical syndromes, patients were grouped as aseptic meningitis (n=654, 52.6%), encephalitis (n=239, 19.2%), febrile seizures (n=153, 12.3%), febrile infant (n=84, 6.7%), neonatal disease (n=70, 5.6%),), acute flaccid paralysis (n=31, 2.4%) and acute disseminated encephalomyelitis (n=11, 0.9%). HEV was detected in 335/1242 CSF samples (26.97%) and was associated to aseptic meningitis (n=243, 72.5%); febrile infant (n=31, 9.2%); neonatal infection (n=26, 7.7%); encephalitis (n=25, 7.5%), febrile seizures (n=9, 2.68%); acute flaccid paralysis (n=1, 0.3%). Seasonal incidence of HEV-B species was analyzed showing that in Buenos Aires infections occur mainly during late spring and summer. Molecular serotyping was completed in 60/335 samples. Echovirus 30, Echovirus 9, Coxsackie B3 to B5 and Echovirus 33 were the most frequently identified.
We showed that HEV are responsible for a considerable proportion of hospitalizations in children with central nervous system compromise reaching 27% of overall etiology.
人类肠道病毒是儿科中枢神经系统（CNS）感染的主要病因之一。
我们研究了1998年1月至2003年12月期间1242名15岁以下疑似中枢神经系统感染的儿童。采集脑脊液，并通过逆转录聚合酶链反应（RT-PCR）和对VP1基因N端部分进行测序，对B型人类肠道病毒进行分子分型。
根据临床综合征，患者分为无菌性脑膜炎（n = 654，52.6%）、脑炎（n = 239，19.2%）、热性惊厥（n = 153，12.3%）、发热婴儿（n = 84，6.7%）、新生儿疾病（n = 70，5.6%）、急性弛缓性麻痹（n = 31，2.4%）和急性播散性脑脊髓炎（n = 11，0.9%）。在1242份脑脊液样本中的335份（26.97%）检测到人类肠道病毒，且与无菌性脑膜炎（n = 243，72.5%）、发热婴儿（n = 3,1，9.2%）、新生儿感染（n = 26，7.7%）、脑炎（n = 25，7.5%）、热性惊厥（n = 9，2.68%）、急性弛缓性麻痹（n = 1，0.3%）有关。分析了B型人类肠道病毒的季节性发病率，结果显示在布宜诺斯艾利斯，感染主要发生在晚春和夏季。对60/335份样本完成了分子血清分型。埃可病毒30型、埃可病毒9型、柯萨奇B3至B5型和埃可病毒33型是最常鉴定出的类型。
我们表明，人类肠道病毒导致相当比例的中枢神经系统受损儿童住院，占总体病因中的27%。
