Tesoriere L, Allegra M, Butera D, Gentile C, Livrea M A
Dipartimento Farmacochimico Tossicologico e Biologico, Università di Palermo, Via Archirafi 32, Palermo 90128, Italy.
Free Radic Res. 2006 Jul;40(7):753-61. doi: 10.1080/10715760600554228.
Antioxidant phytochemicals are investigated as novel treatments for supportive therapy in beta-thalassemia. The dietary indicaxanthin was assessed for its protective effects on human beta-thalassemic RBCs submitted in vitro to oxidative haemolysis by cumene hydroperoxide. Indicaxanthin at 1.0-10 microM enhanced the resistance to haemolysis dose-dependently. In addition, it prevented lipid and haemoglobin (Hb) oxidation, and retarded vitamin E and GSH depletion. After ex vivo spiking of blood from thalassemia patients with indicaxanthin, the phytochemical was recovered in the soluble cell compartment of the RBCs. A spectrophotometric study showed that indicaxanthin can reduce perferryl-Hb generated in solution from met-Hb and hydrogen peroxide (H2O2), more effectively than either Trolox or vitamin C. Collectively our results demonstrate that indicaxanthin can be incorporated into the redox machinery of beta-thalassemic RBC and defend the cell from oxidation, possibly interfering with perferryl-Hb, a reactive intermediate in the hydroperoxide-dependent Hb degradation. Opportunities of therapeutic interest for beta-thalassemia may be considered.
抗氧化植物化学物质被作为β地中海贫血支持性治疗的新方法进行研究。对膳食中的紫黄质进行了评估，以确定其对体外经氢过氧化异丙苯诱导氧化溶血的人β地中海贫血红细胞的保护作用。1.0 - 10微摩尔的紫黄质剂量依赖性地增强了对溶血的抵抗力。此外，它还能防止脂质和血红蛋白（Hb）氧化，并延缓维生素E和谷胱甘肽（GSH）的消耗。在对地中海贫血患者的血液进行紫黄质体外加样后，这种植物化学物质在红细胞的可溶性细胞部分中被检测到。一项分光光度研究表明，紫黄质比Trolox或维生素C更有效地还原了溶液中由高铁血红蛋白（met-Hb）和过氧化氢（H2O2）生成的高铁血红素-Hb。我们的研究结果共同表明，紫黄质可以纳入β地中海贫血红细胞的氧化还原机制，保护细胞免受氧化，可能是通过干扰高铁血红素-Hb（氢过氧化物依赖性Hb降解中的一种反应中间体）来实现的。β地中海贫血治疗方面的潜在机会值得考虑。
