Semenyuk Andrey, Földesi Andras, Johansson Tommy, Estmer-Nilsson Camilla, Blomgren Peter, Brännvall Mathias, Kirsebom Leif A, Kwiatkowski Marek
Department of Genetics and Pathology, Uppsala University, Sweden.
J Am Chem Soc. 2006 Sep 27;128(38):12356-7. doi: 10.1021/ja0636587.
tert-Butyldithiomethyl (DTM), a novel hydroxyl protecting group, cleavable under reductive conditions, was developed and applied for the protection of 2'-OH during solid-phase RNA synthesis. This function is compatible with all standard protecting groups used in oligonucleotide synthesis, and allows for fast and high-yield synthesis of RNA. Oligonucleotides containing the 2'-O-DTM groups can be easily deprotected under the mildest possible aqueous and homogeneous conditions. The preserved 5'-O-DMTr function can be used for high-throughput cartridge RNA purification.
叔丁基二硫甲基（DTM）是一种新型的羟基保护基团，可在还原条件下裂解，已被开发并应用于固相RNA合成过程中2'-OH的保护。该功能与寡核苷酸合成中使用的所有标准保护基团兼容，并允许快速高产率地合成RNA。含有2'-O-DTM基团的寡核苷酸可以在尽可能温和的水性和均相条件下轻松脱保护。保留的5'-O-DMTr功能可用于高通量柱式RNA纯化。
