Kruse Charli, Bodó Enikö, Petschnik Anna E, Danner Sandra, Tiede Stephan, Paus Ralf
Fraunhofer-Institute of Biomedical Engineering, Group of Cell Differentiation and Cell Technology at the University of Lübeck, MFC Innovationscampus, Maria-Goeppert-Strasse 1, D-23538 Lübeck, Germany.
Exp Dermatol. 2006 Oct;15(10):794-800. doi: 10.1111/j.1600-0625.2006.00471.x.
Nestin+ hair follicle-associated cells of murine skin can be isolated and differentiated in vitro into neuronal and glial cells. Therefore, we have asked whether human skin also contains nestin+ cells, and whether these can be differentiated in vitro into neuronal and/or glial cell populations. In this methodological pilot study, we show that both are indeed the case - employing purposely only very simple techniques for isolating, propagating, and differentiating nestin+ cells from normal human scalp skin and its appendages that do not require selective microdissection and tissue compartment isolation prior to cell culture. We show that, it is in principle, possible to maintain and propagate human skin nestin+ cells for extended passage numbers and to differentiate them into both neuronal (i.e. neurofilament+ and/or PGP9.5+) and glial (i.e. GFAP+, MBP+ and/or O4+) cell populations. Therefore, human scalp skin can serve as a highly accessible, abundant, and convenient source for autologous adult stem cell-like cells that offer themselves to be exploited for neuroregenerative medicine purposes.
小鼠皮肤中与毛囊相关的巢蛋白阳性（Nestin+）细胞能够在体外分离并分化为神经元细胞和神经胶质细胞。因此，我们探究了人类皮肤中是否也存在Nestin+细胞，以及这些细胞能否在体外分化为神经元细胞群和/或神经胶质细胞群。在这项方法学初步研究中，我们证实这两个问题的答案都是肯定的——我们特意采用了非常简单的技术，从正常人类头皮皮肤及其附属器中分离、扩增并分化Nestin+细胞，这些技术在细胞培养前无需进行选择性显微切割和组织区室分离。我们表明，原则上有可能维持并扩增人类皮肤Nestin+细胞至较高传代数，并将它们分化为神经元细胞群（即神经丝蛋白阳性和/或PGP9.5阳性）和神经胶质细胞群（即胶质纤维酸性蛋白阳性、髓鞘碱性蛋白阳性和/或O4阳性）。因此，人类头皮皮肤可作为一种极易获取、丰富且便利的自体成体干细胞样细胞来源，有望用于神经再生医学目的。
