Laboratory of Cellular and Molecular Immunobiology of the Skin, Division of Immunology, Allergy and Infectious Diseases, Department of Dermatology, Medical University of Vienna, Vienna, Austria.
J Invest Dermatol. 2012 Mar;132(3 Pt 1):563-74. doi: 10.1038/jid.2011.355. Epub 2011 Nov 3.
Multipotent mesenchymal stromal cells (MSCs) are found in a variety of adult tissues including human dermis. These MSCs are morphologically similar to bone marrow-derived MSCs, but are of unclear phenotype. To shed light on the characteristics of human dermal MSCs, this study was designed to identify and isolate dermal MSCs by a specific marker expression profile, and subsequently rate their mesenchymal differentiation potential. Immunohistochemical staining showed that MSC markers CD73/CD90/CD105, as well as CD271 and SSEA-4, are expressed on dermal cells in situ. Flow cytometric analysis revealed a phenotype similar to bone marrow-derived MSCs. Human dermal cells isolated by plastic adherence had a lower differentiation capacity as compared with bone marrow-derived MSCs. To distinguish dermal MSCs from differentiated fibroblasts, we immunoselected CD271(+) and SSEA-4(+) cells from adherent dermal cells and investigated their mesenchymal differentiation capacity. This revealed that cells with increased adipogenic, osteogenic, and chondrogenic potential were enriched in the dermal CD271(+) population. The differentiation potential of dermal SSEA-4(+) cells, in contrast, appeared to be limited to adipogenesis. These results indicate that specific cell populations with variable mesenchymal differentiation potential can be isolated from human dermis. Moreover, we identified three different subsets of dermal mesenchymal progenitor cells.
多能间充质基质细胞（MSCs）存在于多种成人组织中，包括人真皮。这些 MSCs 在形态上与骨髓来源的 MSCs 相似，但表型尚不清楚。为了阐明人真皮 MSCs 的特征，本研究旨在通过特定的标记物表达谱来鉴定和分离真皮 MSCs，并随后评估其间充质分化潜能。免疫组织化学染色显示 MSC 标记物 CD73/CD90/CD105 以及 CD271 和 SSEA-4 均在真皮细胞原位表达。流式细胞术分析显示出与骨髓来源的 MSCs 相似的表型。与骨髓来源的 MSCs 相比，通过塑料贴附分离的人真皮细胞具有较低的分化能力。为了将真皮 MSCs 与分化的成纤维细胞区分开来，我们从贴壁的真皮细胞中免疫选择 CD271(+)和 SSEA-4(+)细胞，并研究其间充质分化能力。结果表明，具有增加的成脂、成骨和成软骨潜能的细胞在真皮 CD271(+)群体中得到富集。相比之下，真皮 SSEA-4(+)细胞的分化潜力似乎仅限于成脂分化。这些结果表明，可以从人真皮中分离出具有不同间充质分化潜能的特定细胞群体。此外，我们鉴定了三种不同的真皮间充质祖细胞亚群。
