Drevon-Gaillot Elodie, Perron-Lepage Marie-France, Clément Christian, Burnett Roger
MDS Pharma Services, Preclinical Pathology Department, Les Oncins, 69210 Saint-Germain-Sur-L'Arbresle, France.
Exp Toxicol Pathol. 2006 Nov;58(2-3):77-88. doi: 10.1016/j.etp.2006.07.003. Epub 2006 Sep 18.
This review was performed to assess variations in background observations in cynomolgus macaques (Macaca fascicularis) originating from three breeding centres located in Mauritius, The Philippines and Vietnam. The data and tissue samples from 90 cynomolgus monkeys (approximately evenly distributed between the three sources) comprising the control groups from 11 regulatory toxicology studies were used for this investigation. Clinical data--age, body weight, organ weights, haematology and serum biochemistry--were analyzed. Samples of stomach, colon, kidney, heart, liver, spleen and lung were examined microscopically and graded to characterize the degree of lymphoplasmacytic cell infiltration. The main microscopic origin-related variations concerned the digestive tract, where the lymphoplasmacytic cell infiltration grade was significantly lower (p0.001) in cynomolgus monkeys from Mauritius when compared with those from Asia. Generally, only the antral mucosa of the stomach was infiltrated in cynomolgus monkeys from The Philippines, whereas both the fundic and antral regions were infiltrated in those from Vietnam. The digestive tract infiltration grade was strongly correlated with the mean white blood cell count in monkeys from all three sources. Spiral-shaped bacteria were observed in the stomach of monkeys from all three sources, but their presence did not correlate with the severity of the gastric infiltrate. Helicobacter heilmannii-type bacteria were almost always seen in the fundus, Helicobacter pylori-type bacteria were only occasionally seen in the antral region. The incidences of other microscopic findings, such as urothelial cytoplasmic inclusions or Balantidium coli in the caecum, also varied according to the source of the monkeys. Some variations in relative organ weights, haematology and serum biochemistry were also related to the origin of the monkeys, but these did not correlate with the microscopic findings.
本综述旨在评估源自毛里求斯、菲律宾和越南三个繁殖中心的食蟹猴（猕猴）背景观察结果的差异。本研究使用了来自11项监管毒理学研究对照组的90只食蟹猴（三个来源大致均匀分布）的数据和组织样本。分析了临床数据——年龄、体重、器官重量、血液学和血清生化指标。对胃、结肠、肾、心、肝、脾和肺的样本进行显微镜检查并分级，以表征淋巴浆细胞浸润程度。主要的与微观起源相关的差异涉及消化道，与来自亚洲的食蟹猴相比，毛里求斯的食蟹猴淋巴浆细胞浸润分级显著更低（p<0.001）。一般来说，菲律宾的食蟹猴只有胃窦黏膜有浸润，而越南的食蟹猴胃底和胃窦区域均有浸润。来自所有三个来源的猴子消化道浸润分级与平均白细胞计数密切相关。在来自所有三个来源的猴子胃中均观察到螺旋形细菌，但其存在与胃浸润的严重程度无关。海尔曼螺杆菌型细菌几乎总是见于胃底，幽门螺杆菌型细菌仅偶尔见于胃窦区域。其他微观发现的发生率，如盲肠中的尿路上皮细胞质包涵体或结肠小袋纤毛虫，也因猴子的来源不同而有所差异。相对器官重量、血液学和血清生化指标的一些差异也与猴子的来源有关，但这些差异与微观发现无关。
Zotero 插件