主要组织相容性基因和抗原递送对用重组裂殖子抗原免疫后诱导针对堆型艾美耳球虫的保护性粘膜免疫的影响。
Effects of major histocompatibility genes and antigen delivery on induction of protective mucosal immunity to E. acervulina following immunization with a recombinant merozoite antigen.
作者信息
Lillehoj H S, Jenkins M C, Bacon L D
机构信息
Protozoan Diseases Laboratory, U.S. Department of Agriculture, BARC-East, Beltsville, Maryland 20705.
出版信息
Immunology. 1990 Sep;71(1):127-32.
Intramuscular immunization with the recombinant p250 surface antigen of Eimeria acervulina merozoite (rEAMZp250) or oral inoculation with live recombinant Escherichia coli expressing the rEAMZp250 protein resulted in antigen-specific T-cell and humoral responses and conferred a significant reduction in mucosal parasitism compared to immunization with the negative control antigen preparation. Among the major histocompatibility complex (MHC) (B)-congenic chickens receiving intramuscular immunization, strain .6-2 (B2-B2) showed significant (P less than 0.05) protection to live E. acervulina challenge compared to the other strains examined. In contrast, strains .C-12(B12B12) and .P-13 (B13B13) showed significant protection among the groups given live recombinant E. coli. In general, strains showing enhanced T-cell responses to the rEAMZp250 protein were better protected compared to those showing minimal or no T-cell responses. Thus the results suggest that the B haplotypes of the host and the mode of antigen presentation influence the outcome of protection following an immunization of chickens with recombinant coccidial antigen.
用堆型艾美耳球虫裂殖子重组p250表面抗原（rEAMZp250）进行肌肉注射免疫，或用表达rEAMZp250蛋白的重组活大肠杆菌进行口服接种，与用阴性对照抗原制剂免疫相比，可引发抗原特异性T细胞和体液反应，并显著减少黏膜寄生虫感染。在接受肌肉注射免疫的主要组织相容性复合体（MHC）（B）同基因鸡中，与所检测的其他品系相比，品系.6 - 2（B2 - B2）对堆型艾美耳球虫活虫攻击显示出显著（P小于0.05）的保护作用。相比之下，在给予重组活大肠杆菌的组中，品系.C - 12（B12B12）和.P - 13（B13B13）显示出显著的保护作用。总体而言，与那些显示出最小或无T细胞反应的品系相比，对rEAMZp250蛋白显示出增强T细胞反应的品系受到的保护更好。因此，结果表明宿主的B单倍型和抗原呈递方式会影响用重组球虫抗原免疫鸡后保护作用的结果。
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