Egelrud T, Lundström A
Department of Dermatology, University Hospital, Umeå, Sweden.
J Invest Dermatol. 1990 Oct;95(4):456-9. doi: 10.1111/1523-1747.ep12555620.
We have recently shown that cell cohesion in plantar stratum corneum is mediated to a significant extent by protein structures, and that endogenous proteolysis plays an important role in desquamation in this tissue. This paper is a report of our investigations into whether similar mechanisms for cell cohesion and desquamation can be found in non-palmo-plantar stratum corneum. Biopsies of non-palmo-plantar human skin were incubated at 37 degrees C, pH 8, in a buffer with and without additions of detergents (a mixture of N,N-dimethyldodecylamine oxide and sodium dodecyl sulphate), ethylene diamine tetraacetate (EDTA), and the proteinase inhibitor aprotinin. Released cells were examined by phase contrast microscopy and counted. The incubated biopsies were examined by light microscopy. As has been previously shown by others, we found that in the presence of detergents there was a dissociation of stratum corneum cells. This dissociation was stimulated by EDTA and inhibited by aprotinin. After 36 h of incubation the entire stratum corneum and, on some parts of the biopsies, the stratum granulosum had dissociated. There was no evidence of cell dissociation in the spinous or basal epidermal layers. We conclude that the detergent-induced cell dissociation in non-palmo-plantar human stratum corneum is dependent on the action of proteinases present in the tissue on protein structures. These structures may be of significant importance for non-palmo-plantar stratum corneum cell cohesion.
我们最近发现，足底角质层中的细胞黏附在很大程度上是由蛋白质结构介导的，并且内源性蛋白水解在该组织的脱屑过程中起着重要作用。本文报告了我们对非掌跖角质层中是否能发现类似的细胞黏附和脱屑机制的研究。将非掌跖部位的人体皮肤活检组织在37摄氏度、pH值为8的缓冲液中孵育，缓冲液中添加或不添加去污剂（N,N - 二甲基十二烷基氧化胺和十二烷基硫酸钠的混合物）、乙二胺四乙酸（EDTA）以及蛋白酶抑制剂抑肽酶。通过相差显微镜检查并计数释放出的细胞。对孵育后的活检组织进行光学显微镜检查。正如其他人之前所表明的，我们发现在存在去污剂的情况下，角质层细胞会发生解离。这种解离受到EDTA的刺激，并被抑肽酶抑制。孵育36小时后，整个角质层以及活检组织某些部位的颗粒层都发生了解离。在棘层或基底层表皮中没有细胞解离的迹象。我们得出结论，去污剂诱导的非掌跖人体角质层细胞解离取决于组织中存在的蛋白酶对蛋白质结构的作用。这些结构可能对非掌跖角质层细胞黏附具有重要意义。
