Sanli-Yurudu Nazmiye Ozlem, Kimiran-Erdem Ayten, Cotuk Aysin
Department of Biology, Faculty of Science, Istanbul University, 34418 Istanbul, Turkey.
Int J Hyg Environ Health. 2007 Mar;210(2):147-53. doi: 10.1016/j.ijheh.2006.08.004. Epub 2006 Sep 20.
Effectiveness of Chloramine T trihydrate (N-chloro-p-toluene sulfonamide) on both planktonic and sessile populations of different Legionella pneumophila strains was assessed. Although Chloramine T is a recommended commercial formulation for disinfecting cooling towers, there is a lack of published data about the efficacy of this compound against both planktonic and sessile populations of L. pneumophila. Planktonic L. pneumophila strains were suspended in tap water and sessile L. pneumophila strains were grown on stainless steel which is used in the construction of cooling towers, followed by exposure to the biocide. The sensitivity of both planktonic and sessile populations of L. pneumophila strains was different. The biocide was found effective below recommended dosages (0.1-0.3%) against planktonic populations of L. pneumophila, whereas it was determined that higher dosages than those recommended were required for sessile populations of L. pneumophila. The results indicated that studying only the planktonic populations of L. pneumophila for biocide tests might not be sufficient to provide information about the optimum dosage and contact time. Therefore, efficacy has to be tested on both planktonic and sessile bacteria.
评估了三水合氯胺T（N-氯对甲苯磺酰胺）对不同嗜肺军团菌菌株的浮游菌和固着菌群体的有效性。尽管氯胺T是一种推荐用于冷却塔消毒的商业制剂，但关于该化合物对嗜肺军团菌浮游菌和固着菌群体的功效，缺乏已发表的数据。将嗜肺军团菌浮游菌菌株悬浮于自来水中，将嗜肺军团菌固着菌菌株培养在冷却塔建造中使用的不锈钢上，然后使其接触杀菌剂。嗜肺军团菌菌株的浮游菌和固着菌群体的敏感性不同。发现该杀菌剂在低于推荐剂量（0.1 - 0.3%）时对嗜肺军团菌浮游菌群体有效，而对于嗜肺军团菌固着菌群体，确定需要高于推荐剂量的剂量。结果表明，仅研究嗜肺军团菌浮游菌群体进行杀菌剂测试可能不足以提供关于最佳剂量和接触时间的信息。因此，必须对浮游菌和固着菌都进行功效测试。
