Gonçalves A M, Nehme N S, Morel C M
Instituto Oswaldo Cruz, Departamento de Bioquímica e Biologia Molecular, Rio de Janeiro, Brasil.
Mem Inst Oswaldo Cruz. 1990 Jan-Mar;85(1):101-6. doi: 10.1590/s0074-02761990000100017.
A simple protocol is described for the silver staining of polyacrylamide gradient gels used for the separation of restriction fragments of kinetoplast DNA [schizodeme analysis of trypanosomatids (Morel et al., 1980)]. The method overcomes the problems of non-uniform staining and strong background color which are frequently encountered when conventional protocols for silver staining of linear gels are applied to gradient gels. The method described has proven to be of general applicability for DNA, RNA and protein separations in gradient gels.
本文描述了一种简单的protocol，用于对用于分离动质体DNA限制性片段的聚丙烯酰胺梯度凝胶进行银染[锥虫的裂殖体分析（莫雷尔等人，1980年）]。该方法克服了将线性凝胶银染的传统protocol应用于梯度凝胶时经常遇到的染色不均匀和背景颜色深的问题。所描述的方法已被证明普遍适用于梯度凝胶中的DNA、RNA和蛋白质分离。
