Miller Ingrid, Crawford Johanne, Gianazza Elisabetta
Institut für Medizinische Chemie, Department für Naturwissenschaften, Veterinärmedizinische Universität Wien, Wien, Austria.
Proteomics. 2006 Oct;6(20):5385-408. doi: 10.1002/pmic.200600323.
This review recollects literature data on sensitivity and dynamic range for the most commonly used colorimetric and fluorescent dyes for general protein staining, and summarizes procedures for the most common PTM-specific detection methods. It also compiles some important points to be considered in imaging and evaluation. In addition to theoretical considerations, examples are provided to illustrate differential staining of specific proteins with different detection methods. This includes a large body of original data on the comparative evaluation of several pre- and post-electrophoresis stains used in parallel on a single specimen, horse serum run in 2-DE (IPG-DALT). A number of proteins/protein spots are found to be over- or under-revealed with some of the staining procedures.
本综述回顾了有关用于一般蛋白质染色的最常用比色染料和荧光染料的灵敏度和动态范围的文献数据，并总结了最常见的特定翻译后修饰（PTM）检测方法的步骤。它还汇编了成像和评估中需要考虑的一些要点。除了理论考量外，还提供了示例来说明使用不同检测方法对特定蛋白质进行差异染色的情况。这包括大量关于在单个样本上并行使用的几种电泳前和电泳后染色的比较评估的原始数据，该样本为在二维电泳（IPG-DALT）中运行的马血清。发现一些染色程序会使许多蛋白质/蛋白斑点显示过度或不足。
