van der Velden L A, Schaafsma H E, Manni J J, Ruiter D J, Ramaekers F C, Kuijpers W
Department of Otorhinolaryngology, University Hospital Nijmegen, The Netherlands.
Eur Arch Otorhinolaryngol. 1997;254(8):376-83. doi: 10.1007/BF01642554.
The immunohistochemical expression patterns of cytokeratin polypeptides and vimentin were investigated in normal epithelia and squamous cell carcinomas of the larynx with special emphasis on tumor grading. During malignant transformation of epithelial cells, the cytokeratin expression patterns changed, depending on the differentiation grade of the carcinomas. In low-grade carcinomas, the expression patterns were close to those of the normal epithelium. With increasing tumor grade, there was decreased expression of stratification cytokeratins and increased expression of basal cell, simple cell and hyperproliferation-related cytokeratins. Increasing tumor grade was also associated with the expression of vimentin, a cytoskeletal protein of mesenchymal cells. No relationship was found between vimentin expression and the presence of lymph-node metastases.
研究了细胞角蛋白多肽和波形蛋白在正常喉上皮和喉鳞状细胞癌中的免疫组化表达模式，特别关注肿瘤分级。在上皮细胞恶性转化过程中，细胞角蛋白的表达模式根据癌的分化程度而改变。在低级别癌中，表达模式与正常上皮相似。随着肿瘤级别的增加，分层细胞角蛋白的表达减少，基底细胞、单细胞和与过度增殖相关的细胞角蛋白的表达增加。肿瘤级别增加还与波形蛋白（一种间充质细胞的细胞骨架蛋白）的表达有关。未发现波形蛋白表达与淋巴结转移之间存在关联。
