Vijayan V, Quak S H, Wong H B
Department of Paediatrics, National University Hospital, Singapore.
Ann Trop Paediatr. 1990;10(2):179-83. doi: 10.1080/02724936.1990.11747427.
Two hundred and eighteen patients with acute gastro-enteritis (GE) and 67 controls (patients admitted during the period of study for illness other than (GE) were included in this study. Their stool samples were subjected to the following tests to detect the presence of rotavirus: enzyme-linked immunosorbent assay (ELISA), latex agglutination and electron microscopy. Samples positive by any one or more of the above methods were considered positive for rotavirus and were subjected to polyacrylamide gel electrophoresis of viral RNA. Rotavirus was detected in 59 (27.1%) of the 218 GE samples and four (6%) of the control samples. ELISA was the most sensitive test and could detect viral antigen long after the other methods failed to do so.
本研究纳入了218例急性胃肠炎（GE）患者和67名对照（研究期间因GE以外疾病入院的患者）。对他们的粪便样本进行以下检测以检测轮状病毒的存在：酶联免疫吸附测定（ELISA）、乳胶凝集试验和电子显微镜检查。通过上述任何一种或多种方法检测呈阳性的样本被视为轮状病毒阳性，并进行病毒RNA的聚丙烯酰胺凝胶电泳。在218份GE样本中有59份（27.1%）检测到轮状病毒，在对照样本中有4份（6%）检测到轮状病毒。ELISA是最敏感的检测方法，在其他方法无法检测到病毒抗原后很长时间仍能检测到。
