el Assouli S M, Banjar Z M, Mohammed K A, Zamakhchari F T
Department of Biology, Faculty of Medicine, King Abdulaziz University, Jeddah, Saudi Arabia.
Am J Trop Med Hyg. 1992 Mar;46(3):272-7. doi: 10.4269/ajtmh.1992.46.272.
Three hundred sixty-three fecal specimens were collected from infants and young children with gastroenteritis over a 13-month period in Jeddah, western Saudi Arabia. Rotavirus was detected in 46% of the 363 specimens tested using an enzyme-linked immunosorbent assay (ELISA), and in 40.7% of 113 specimens using a latex agglutination test. One hundred nine of the 113 specimens that were positive by the latex agglutination test were also positive by ELISA. Electron microscopy was used to examine some specimens to demonstrate the presence of the virus. Rotavirus was detected throughout the 13-month study period, with an increase in the frequency of infection in the cooler months. Infection with this virus was more frequent among infants and children less than two-years old, with a maximum incidence among children 13-15 months old. In the 363 stool specimens tested, rotavirus was found in mixed infections with bacteria in 0.44%, with parasites in 1.31%, and with yeast in 0.66%.
在沙特阿拉伯西部吉达市为期13个月的时间里，从患有肠胃炎的婴幼儿身上采集了363份粪便样本。使用酶联免疫吸附测定法（ELISA）对363份检测样本进行检测，其中46%检测出轮状病毒；使用乳胶凝集试验对113份样本进行检测，40.7%检测出轮状病毒。乳胶凝集试验呈阳性的113份样本中，有109份ELISA检测也呈阳性。使用电子显微镜检查部分样本以证实病毒的存在。在整个13个月的研究期间均检测到轮状病毒，在较凉爽的月份感染频率有所增加。这种病毒在两岁以下的婴幼儿中感染更为频繁，在13 - 15个月大的儿童中发病率最高。在检测的363份粪便样本中，发现轮状病毒与细菌混合感染的占0.44%，与寄生虫混合感染的占1.31%，与酵母菌混合感染的占0.66%。
