Lourenco M H, Thompson R, Drucker J, Grenier B
Pathol Biol (Paris). 1985 Feb;33(2):93-7.
From January first 1978 to September 30, 1981, 93 fecal samples from children hospitalized with a rotavirus gastro-enteritis were tested after RNA extraction and polyacrylamide gel electrophoresis. 14 electrophoretypes (TI--T14) were identified by this technique. They were studied in relation with epidemiological as well as clinical symptoms. No electrophoretype correlated well with age or living area. The electrophoretype T3 was found only in male infants and the electrophoretype T7 was 3 times more frequent in boys than in girls. The electrophoretype T4 was prevailing during the winter 1980-1981 and the electrophoretype T5 prevailed during the summer 1981. 19 infants were probably contaminated during their stay in the hospital, 9 of them with the electrophoretype T4 prevailing at that time. The prevalence of associated otitis and rash was significantly higher with the electrophoretype T3. The polyacrylamide gel electrophoresis technique appears to be a simple, and reliable epidemiological tool to evaluate rotavirus infection in communities.
1978年1月1日至1981年9月30日期间，对93例因轮状病毒肠胃炎住院儿童的粪便样本进行了RNA提取和聚丙烯酰胺凝胶电泳检测。通过该技术鉴定出14种电泳类型（TI-T14）。对它们与流行病学及临床症状的关系进行了研究。没有一种电泳类型与年龄或居住地区有很好的相关性。电泳类型T3仅在男婴中发现，电泳类型T7在男孩中的出现频率是女孩的3倍。电泳类型T4在1980-1981年冬季占主导，电泳类型T5在1981年夏季占主导。19名婴儿可能在住院期间受到感染，其中9名感染当时占主导的电泳类型T4。与电泳类型T3相关的中耳炎和皮疹的患病率明显更高。聚丙烯酰胺凝胶电泳技术似乎是一种评估社区轮状病毒感染的简单且可靠的流行病学工具。
