Fetissof F, Serres G, Haillot O
Laboratoire d'Anatomie pathologique, Faculté de Médecine, Tours.
Arch Anat Cytol Pathol. 1990;38(4):159-62.
The purpose of this study was to determine the tissue distribution of certain cytokeratins in the urothelium. A series of ten cystectomy specimens containing normal tissues was investigated by immunocytochemical labeling with specific antibodies against cytokeratins 19, 18, 8, 7, 10. Monoclonal antibodies were used on frozen sections. Distribution of keratin was correlated with morphologic changes. All specimens were lined by a normal transitional epithelium. Low molecular weight cytokeratins (polypeptides 19, 18, 8, 7) were detected in all cell layers. Anticytokeratin 19 stained epithelium in a rather uniform way. Different antibodies specific for cytokeratin 18 reacted differently on the same tissue. With certain anticytokeratins 18 and 8, a higher staining intensity was found in superficial layers as compared with intermediate cell layers. So-called "umbrella" cells were strongly stained. Anticytokeratin 10 revealed very rare endocrine-like cells. This normal urothelial pattern is in agreement with previous reports. This pattern of expression of cytokeratin polypeptides differs from that of nonkeratinizing squamous epithelium. Therefore, histological differentiation of epithelia is accompanied by pronounced changes in the expression of cytokeratin polypeptides.
本研究的目的是确定某些细胞角蛋白在尿路上皮中的组织分布。通过用针对细胞角蛋白19、18、8、7、10的特异性抗体进行免疫细胞化学标记，对一系列包含正常组织的十个膀胱切除术标本进行了研究。单克隆抗体用于冰冻切片。角蛋白的分布与形态学变化相关。所有标本均内衬正常移行上皮。在所有细胞层中均检测到低分子量细胞角蛋白（多肽19、18、8、7）。抗细胞角蛋白19以相当均匀的方式对上皮进行染色。针对细胞角蛋白18的不同抗体在同一组织上反应不同。使用某些抗细胞角蛋白18和8时，与中间细胞层相比，表层的染色强度更高。所谓的“伞状”细胞被强烈染色。抗细胞角蛋白10显示出非常罕见的内分泌样细胞。这种正常的尿路上皮模式与先前的报道一致。这种细胞角蛋白多肽的表达模式与非角化鳞状上皮的表达模式不同。因此，上皮的组织学分化伴随着细胞角蛋白多肽表达的明显变化。
