Ramaekers F, Huysmans A, Moesker O, Schaart G, Herman C, Vooijs P
Lab Invest. 1985 Jan;52(1):31-8.
Antibodies to intermediate filament proteins were used to study different cell layers in normal human transitional epithelium, 16 human transitional cell carcinomas, and two cell lines derived from human bladder carcinomas. Conventional rabbit antisera to human skin keratins stained all layers of the transitional epithelium from bladder, ureter, and kidney. A slightly higher staining intensity was found in the basal and superficial layers as compared with the intermediate cell layers. A monoclonal antibody to cytokeratin 18 (RGE 53), however, stained only the superficial cell layer of transitional epithelium, the so-called umbrella cells. In well-differentiated (grade I) transitional cell carcinomas, RGE 53 stained only the superficial cells of papillary structures. In higher grade papillary tumors, RGE 53 also stained cells within the basal and intermediate layers, whereas in high-grade, invasive tumors almost all tumor cells were RGE 53 positive. These results show that monoclonal antibodies to cytokeratins can provide both an indication of processes involved in neoplastic progression of bladder tumors and a means of studying the molecular relationship of the tumor cells to normal cells.
使用针对中间丝蛋白的抗体来研究正常人类移行上皮、16例人类移行细胞癌以及两种源自人类膀胱癌的细胞系中的不同细胞层。传统的针对人类皮肤角蛋白的兔抗血清可对膀胱、输尿管和肾脏的移行上皮各层进行染色。与中间细胞层相比，在基底层和表层发现染色强度略高。然而，一种针对细胞角蛋白18的单克隆抗体（RGE 53）仅对移行上皮的表层细胞，即所谓的伞细胞进行染色。在高分化（I级）移行细胞癌中，RGE 53仅对乳头状结构的表层细胞进行染色。在更高分级的乳头状肿瘤中，RGE 53也对基底层和中间层的细胞进行染色，而在高级别浸润性肿瘤中，几乎所有肿瘤细胞均为RGE 53阳性。这些结果表明，针对细胞角蛋白的单克隆抗体既可以指示膀胱肿瘤肿瘤进展过程中涉及的过程，也可以作为研究肿瘤细胞与正常细胞分子关系的一种手段。
