Valentin A, Matsuda S, Asjo B
Department of Virology, Karolinska Institute, Stockholm, Sweden.
AIDS Res Hum Retroviruses. 1990 Aug;6(8):977-8. doi: 10.1089/aid.1990.6.977.
The in vitro maturation of peripheral blood monocytes to macrophages can be followed morphologically, and by measurement of cell surface antigens (CD4, HLA-DR, and FcR III) and lysozyme production. We used these markers to correlate monocyte maturation with susceptibility to human immunodeficiency virus (HIV) infection. Maturation of peripheral blood monocytes is associated with a decrease in membrane CD4, while HLA-DR and FcR III expression increase along with lysozyme secretion. Cells at all stages of maturation were susceptible to HIV infection, even mature macrophages without CD4 detectably by immunofluorescent staining. Maximal replication was observed in 7-day-old cells.
外周血单核细胞向巨噬细胞的体外成熟过程可以通过形态学观察，以及通过测量细胞表面抗原（CD4、HLA-DR和FcR III）和溶菌酶产生来追踪。我们使用这些标志物来关联单核细胞成熟与对人类免疫缺陷病毒（HIV）感染的易感性。外周血单核细胞的成熟与膜CD4的减少相关，而HLA-DR和FcR III的表达随着溶菌酶分泌而增加。所有成熟阶段的细胞都易受HIV感染，即使是通过免疫荧光染色无法检测到CD4的成熟巨噬细胞也是如此。在7日龄的细胞中观察到最大复制。
