Jang Junho, Yotsu-Yamashita Mari
Graduate School of Agricultural Science, Tohoku University, 1-1 Tsutsumidori-Amamiyamachi, Aoba-ku, Sendai 981-8555, Japan.
Toxicon. 2006 Dec 15;48(8):980-7. doi: 10.1016/j.toxicon.2006.07.034. Epub 2006 Aug 14.
The anatomical distribution of tetrodotoxin (TTX), saxitoxin (STX) and their analogs (TTXs, STXs) in three female and three male specimens of the marine puffer fish Fugu pardalis from Miyagi Prefecture, 2005, Japan, were studied. 5-DeoxyTTX, 11-deoxyTTX, and 5,6,11-trideoxyTTX were quantified by liquid chromatography/mass spectrometry (LC/MS) for the first time, and other TTXs and STXs were determined by liquid chromatography-fluorescent detection (LC-FLD). As a result, 5,6,11-trideoxyTTX was found to be the major TTX analog in all tissues tested, whereas 5-deoxyTTX and 11-deoxyTTX were minor components. Especially, in female (n=3), the ratios of 5,6,11-trideoxyTTX to total of all TTX analogs (mole/mole) in ovaries (mean+/-SD, 0.42+/-0.055) were significantly larger than those in livers (0.17+/-0.025) (P<0.05). In contrary, the ratios of 4,9-anhydroTTX to total of all TTX analogs in livers (0.27+/-0.047) were significantly larger than those in ovaries (0.073+/-0.040) (P<0.01). The ratios of TTX to total of all TTX analogs were not significantly different between ovaries (0.47+/-0.078) and livers (0.55+/-0.067). In male (n=3), all these ratios were not significantly different between livers and testis. 4-S-CysteinylTTX was detected in liver, spleen, gall, and intestine in 1-6mole% of total of all TTX analogs, supporting our previous hypothesis that 4-S-cysteinylTTX is a metabolite of TTX.
2005年，对来自日本宫城县的3只雌性和3只雄性豹纹东方鲀（Fugu pardalis）体内河豚毒素（TTX）、石房蛤毒素（STX）及其类似物（TTXs、STXs）的解剖分布进行了研究。首次通过液相色谱/质谱联用（LC/MS）对5-脱氧TTX、11-脱氧TTX和5,6,11-三脱氧TTX进行了定量分析，其他TTXs和STXs则通过液相色谱-荧光检测（LC-FLD）进行测定。结果显示，5,6,11-三脱氧TTX是所有测试组织中的主要TTX类似物，而5-脱氧TTX和11-脱氧TTX则是次要成分。特别是在雌性个体（n = 3）中，卵巢中5,6,11-三脱氧TTX与所有TTX类似物总量的比例（平均值±标准差，0.42±0.055）显著高于肝脏中的比例（0.17±0.025）（P < 0.05）。相反，肝脏中4,9-脱水TTX与所有TTX类似物总量的比例（0.27±0.047）显著高于卵巢中的比例（0.073±0.040）（P < 0.01）。卵巢（0.47±0.078）和肝脏（0.55±0.067）中TTX与所有TTX类似物总量的比例没有显著差异。在雄性个体（n = 3）中，肝脏和睾丸之间的所有这些比例均无显著差异。在肝脏、脾脏、胆囊和肠道中检测到4-S-半胱氨酸基TTX，占所有TTX类似物总量的1 - 6摩尔%，这支持了我们之前关于4-S-半胱氨酸基TTX是TTX代谢产物的假设。
