来自大肠杆菌需氧苯乙酸降解途径的苯乙酰辅酶A加氧酶的遗传特征分析
Genetic characterization of the phenylacetyl-coenzyme A oxygenase from the aerobic phenylacetic acid degradation pathway of Escherichia coli.
作者信息
Fernández Cristina, Ferrández Abel, Miñambres Baltasar, Díaz Eduardo, García José L
机构信息
Department of Molecular Microbiology, Centro de Invetigaciones Biológicas, Consejo Superior de Investigaciones Científicas, Ramiro de Maeztu 9, 28040 Madrid, Spain.
出版信息
Appl Environ Microbiol. 2006 Nov;72(11):7422-6. doi: 10.1128/AEM.01550-06. Epub 2006 Sep 22.
Abstract
We show here that the paaABCDE genes of the paa cluster responsible for phenylacetate degradation in Escherichia coli W encode a five-component oxygenase that hydroxylates phenylacetyl-coenzyme A (CoA), the first intermediate of the pathway. The primary structure of the subunits of bacterial phenylacetyl-CoA oxygenases revealed that these enzymes constitute the prototype of a new and distinct group of the large bacterial diiron multicomponent oxygenase family.
摘要
我们在此表明，大肠杆菌W中负责苯乙酸降解的paa操纵子的paaABCDE基因编码一种五组分加氧酶，该酶可将该途径的首个中间产物苯乙酰辅酶A（CoA）羟基化。细菌苯乙酰辅酶A加氧酶亚基的一级结构表明，这些酶构成了大型细菌双铁多组分加氧酶家族中一个新的独特组的原型。
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