Das S N, Paul B N, Saxena A K, Ray P K
Immunobiology Division, Industrial Toxicology Research Centre, M.G. Marg, Lucknow, India.
Immunopharmacol Immunotoxicol. 1990;12(2):293-310. doi: 10.3109/08923979009019674.
Gestational exposure to 10 and 100 mg/kg body weight (m.b.w.) hexachlorocyclohexane throughout the gestation period was done to Swiss albino mice. HCH (alpha, beta and gamma isomers) residue analysis in pups showed a higher contamination in the lymphoid organs (Spleen, Thymus and Kidney) than liver in dose dependent manner. Immune functions of the offsprings of these dams along with the offsprings of vehicle treated or untreated control dams were assessed using selected parameters of both the cellular and humoral immune responses. The delayed hypersensitivity (DTH) response to sheep erythrocytes (SRBC) was significantly higher (p less than 0.01) in pups of the dams exposed to 10 mg/kg b.w. HCH and significantly impaired in pups of the dams exposed to 100 mg/kg m.b.w. HCH as compared to controls. Mitogenic responsiveness of the spleen cells in response to Concanavalin A (Con A) and Lipopolysaccharide (LPS) was almost two fold and eight fold higher respectively and antibody response to SRBC, as measured by plaque forming cells (PFC) assay was two fold higher (p less than 0.001) in pups exposed to 10 mg/kg HCH. However, 100 mg/kg m.b.w. HCH did not affect either mitogenic response or PFC response of the pups. The results, therefore, suggest that lower dose of HCH is capable of modulating the development and function of developing immune system possibly by modulating the functional organization of the T-cell populations.
在整个妊娠期，对瑞士白化小鼠给予体重10毫克/千克和100毫克/千克的六氯环己烷。对幼崽体内六氯环己烷（α、β和γ异构体）残留分析显示，淋巴器官（脾脏、胸腺和肾脏）中的污染程度高于肝脏，且呈剂量依赖性。使用细胞免疫和体液免疫反应的选定参数，评估了这些母鼠后代以及接受赋形剂处理或未处理的对照母鼠后代的免疫功能。与对照组相比，暴露于10毫克/千克体重六氯环己烷的母鼠所产幼崽对绵羊红细胞（SRBC）的迟发型超敏反应（DTH）显著更高（p<0.01），而暴露于100毫克/千克体重六氯环己烷的母鼠所产幼崽的DTH反应则显著受损。脾脏细胞对刀豆蛋白A（Con A）和脂多糖（LPS）的促有丝分裂反应分别高出近两倍和八倍，通过空斑形成细胞（PFC）测定法测得，暴露于10毫克/千克六氯环己烷的幼崽对SRBC的抗体反应高出两倍（p<0.001）。然而，100毫克/千克体重的六氯环己烷对幼崽的促有丝分裂反应或PFC反应均无影响。因此，结果表明，较低剂量的六氯环己烷可能通过调节T细胞群体的功能组织，从而调节发育中免疫系统的发育和功能。
