Tian Kan, Chen Hongli, Tang Jianghong, Chen Xingguo, Hu Zhide
Department of Chemistry, Lanzhou University, Lanzhou 730000, China.
J Chromatogr A. 2006 Nov 3;1132(1-2):333-6. doi: 10.1016/j.chroma.2006.08.090. Epub 2006 Sep 26.
The enantioseparation of four stereoisomers of palonosetron hydrochloride by micellar electrokinetic chromatography using sodium cholate as chiral surfactant was described. Sodium cholate was shown to be effective in separating palonosetron hydrochloride stereoisomers. For method optimization, several parameters such as sodium cholate concentration, buffer pH and concentration, the types and concentration of organic modifiers and applied voltage, on the enantioseparation were evaluated and the optimum conditions were obtained as follows: 30 mM borate buffer (pH 9.40) containing 70 mM sodium cholate and 20% (v/v) methanol with an applied voltage of 20 kV. Under these conditions, baseline separation of palonosetron hydrochloride stereoisomers was achieved within 18 min.
描述了使用胆酸钠作为手性表面活性剂，通过胶束电动色谱法对盐酸帕洛诺司琼四种立体异构体进行对映体分离。结果表明，胆酸钠对分离盐酸帕洛诺司琼立体异构体有效。为了优化方法，评估了几个参数，如胆酸钠浓度、缓冲液pH值和浓度、有机改性剂的类型和浓度以及施加电压对对映体分离的影响，并获得了以下最佳条件：30 mM硼酸盐缓冲液（pH 9.40），含有70 mM胆酸钠和20%（v/v）甲醇，施加电压为20 kV。在这些条件下，18分钟内实现了盐酸帕洛诺司琼立体异构体的基线分离。
