Australian Centre for Research on Separation Science (ACROSS), School of Natural Sciences-Chemistry, University of Tasmania, Hobart 7001, Australia.
Molecules. 2021 Sep 12;26(18):5531. doi: 10.3390/molecules26185531.
Bile salts are naturally occurring chiral surfactants that are able to solubilize hydrophobic compounds. Because of this ability, bile salts were exploited as chiral selectors added to the background solution (BGS) in the chiral micellar electrokinetic chromatography (MEKC) of various small molecules. In this review, we aimed to examine the developments in research on chiral MEKC using bile salts as chiral selectors over the past 20 years. The review begins with a discussion of the aggregation of bile salts in chiral recognition and separation, followed by the use of single bile salts and bile salts with other chiral selectors (i.e., cyclodextrins, proteins and single-stranded DNA aptamers). Advanced techniques such as partial-filling MEKC, stacking and single-drop microextraction were considered. Potential applications to real samples, including enantiomeric impurity analysis, were also discussed.
胆盐是天然存在的手性表面活性剂，能够增溶疏水性化合物。由于这种能力，胆盐被用作手性选择剂，添加到背景溶液（BGS）中，用于各种小分子的手性胶束电动色谱（MEKC）中。在这篇综述中，我们旨在检查过去 20 年来使用胆盐作为手性选择剂的手性 MEKC 研究的进展。综述首先讨论了胆盐在手性识别和分离中的聚集，然后讨论了单一胆盐和与其他手性选择剂（即环糊精、蛋白质和单链 DNA 适体）一起使用的胆盐。还考虑了部分填充 MEKC、堆积和单滴微萃取等先进技术。还讨论了其在真实样品中的潜在应用，包括对映体杂质分析。
