高通量技术在细菌和病毒病原体蛋白质结构测定中的应用。
Application of the use of high-throughput technologies to the determination of protein structures of bacterial and viral pathogens.
作者信息
Fogg M J, Alzari P, Bahar M, Bertini I, Betton J M, Burmeister W P, Cambillau C, Canard B, Corrondo M A, Coll M, Daenke S, Dym O, Egloff M P, Enguita F J, Geerlof A, Haouz A, Jones T A, Ma Qingjun, Manicka S N, Migliardi M, Nordlund P, Owens R J, Peleg Y, Schneider G, Schnell R, Stuart D I, Tarbouriech N, Unge T, Wilkinson A J, Wilmanns M, Wilson K S, Zimhony O, Grimes J M
机构信息
York Structural Biology Laboratory, Department of Chemistry, University of York, York, England.
出版信息
Acta Crystallogr D Biol Crystallogr. 2006 Oct;62(Pt 10):1196-207. doi: 10.1107/S0907444906030915. Epub 2006 Sep 19.
The Structural Proteomics In Europe (SPINE) programme is aimed at the development and implementation of high-throughput technologies for the efficient structure determination of proteins of biomedical importance, such as those of bacterial and viral pathogens linked to human health. Despite the challenging nature of some of these targets, 175 novel pathogen protein structures (approximately 220 including complexes) have been determined to date. Here the impact of several technologies on the structural determination of proteins from human pathogens is illustrated with selected examples, including the parallel expression of multiple constructs, the use of standardized refolding protocols and optimized crystallization screens.
欧洲结构蛋白质组学（SPINE）计划旨在开发和应用高通量技术，以高效测定具有生物医学重要性的蛋白质结构，例如与人类健康相关的细菌和病毒病原体的蛋白质结构。尽管其中一些目标具有挑战性，但迄今为止已确定了175种新型病原体蛋白质结构（包括复合物在内约220种）。本文通过选定的实例说明了几种技术对人类病原体蛋白质结构测定的影响，包括多种构建体的平行表达、标准化重折叠方案的使用以及优化的结晶筛选。
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