Institut für Pharmazie und Molekulare Biotechnologie, Universität Heidelberg, 69120 Heidelberg, Germany.
Trends Microbiol. 2011 Oct;19(10):501-8. doi: 10.1016/j.tim.2011.07.003. Epub 2011 Aug 18.
Novel high-throughput technologies such as yeast two-hybrid and RNA interference (RNAi) screens provide the tools to study interactions between viral proteins and the host on a genomic scale. In this review, we provide an overview of studies in which these technologies were applied and of computational approaches for the analysis of the identified viral interactors in the context of the host cell. The results of these studies illustrate the advantages of integrative systems biology approaches in the investigation of viral pathogens.
新型高通量技术，如酵母双杂交和 RNA 干扰 (RNAi) 筛选，为研究病毒蛋白与宿主之间在基因组范围内的相互作用提供了工具。在这篇综述中，我们概述了应用这些技术的研究，并介绍了用于分析宿主细胞中鉴定出的病毒相互作用因子的计算方法。这些研究的结果说明了综合系统生物学方法在研究病毒病原体方面的优势。
