Banci L, Bertini I, Cusack S, de Jong R N, Heinemann U, Jones E Y, Kozielski F, Maskos K, Messerschmidt A, Owens R, Perrakis A, Poterszman A, Schneider G, Siebold C, Silman I, Sixma T, Stewart-Jones G, Sussman J L, Thierry J C, Moras Dino
CIRMMP, CERM, Via Sacconi 6, Sesto Fiorentino, Italy.
Acta Crystallogr D Biol Crystallogr. 2006 Oct;62(Pt 10):1208-17. doi: 10.1107/S0907444906029350. Epub 2006 Sep 19.
The EC 'Structural Proteomics In Europe' contract is aimed specifically at the atomic resolution structure determination of human protein targets closely linked to health, with a focus on cancer (kinesins, kinases, proteins from the ubiquitin pathway), neurological development and neurodegenerative diseases and immune recognition. Despite the challenging nature of the analysis of such targets, approximately 170 structures have been determined to date. Here, the impact of high-throughput technologies, such as parallel expression of multiple constructs, the use of standardized refolding protocols and optimized crystallization screens or the use of mass spectrometry to assist sample preparation, on the structural biology of mammalian protein targets is illustrated through selected examples.
欧盟“欧洲结构蛋白质组学”项目合同专门致力于确定与健康密切相关的人类蛋白质靶点的原子分辨率结构，重点关注癌症（驱动蛋白、激酶、泛素途径中的蛋白质）、神经发育和神经退行性疾病以及免疫识别。尽管分析此类靶点具有挑战性，但迄今为止已确定了约170个结构。本文通过实例展示了高通量技术，如多种构建体的平行表达、标准化重折叠方案和优化的结晶筛选的使用，或利用质谱辅助样品制备，对哺乳动物蛋白质靶点结构生物学的影响。
