de Graeff-Meeder E R, Voorhorst M, van Eden W, Schuurman H J, Huber J, Barkley D, Maini R N, Kuis W, Rijkers G T, Zegers B J
Department of Immunology, Wilhelmina Children's Hospital, Utrecht, The Netherlands.
Am J Pathol. 1990 Nov;137(5):1013-7.
The expression of 65-kd mycobacterial heat-shock protein (HSP)-related antigens in synovial membrane from rats and humans with arthritis was studied using three monoclonal antibodies and one polyclonal antiserum directed to antigens of mycobacteria. The antibodies labeled synovial tissue sections from both adjuvant arthritis (AA) rats and from patients with either rheumatoid arthritis (RA) or osteoarthritis (OA); especially the synovial lining cells appeared to be positive. The cytoplasmic staining patterns in rats and humans were essentially the same and were not related to the extent of inflammation ie, the size of lymphoid infiltration. In control tissues no cytoplasmic staining was observed. The results suggest a role for a 65-kd HSP or a cross-reactive molecule in the immunopathologic process of arthritic disease.
使用三种针对分枝杆菌抗原的单克隆抗体和一种多克隆抗血清，研究了65-kd分枝杆菌热休克蛋白（HSP）相关抗原在患有关节炎的大鼠和人类滑膜中的表达。这些抗体标记了佐剂性关节炎（AA）大鼠以及类风湿性关节炎（RA）或骨关节炎（OA）患者的滑膜组织切片；尤其是滑膜衬里细胞似乎呈阳性。大鼠和人类的细胞质染色模式基本相同，且与炎症程度即淋巴浸润的大小无关。在对照组织中未观察到细胞质染色。结果表明65-kd HSP或一种交叉反应分子在关节炎疾病的免疫病理过程中起作用。
