Muraiso K, Hijikata M, Ohkoshi S, Cho M J, Kikuchi M, Kato N, Shimotohno K
Virology Division, National Cancer Center Research Institute, Tokyo, Japan.
Biochem Biophys Res Commun. 1990 Oct 30;172(2):511-6. doi: 10.1016/0006-291x(90)90702-o.
A putative core protein derived from hepatitis C virus was expressed in E. coli. More than 5% of the total protein expressed in the bacteria after induction by isopropylthio-beta-D-galactoside was shown to be the expected protein. Western blotting with this E. coli lysate proved to be more efficient than ELISA with a non-structural viral protein, C100, to detect infection of hepatitis C virus in the sera of patients with non-A, non-B chronic hepatitis, hepatocellular carcinoma as well as in sera from healthy persons.
一种源自丙型肝炎病毒的假定核心蛋白在大肠杆菌中表达。经异丙基硫代-β-D-半乳糖苷诱导后，细菌中表达的总蛋白有超过5%为预期蛋白。用这种大肠杆菌裂解物进行蛋白质印迹分析，在检测非甲非乙型慢性肝炎患者、肝细胞癌患者以及健康人血清中的丙型肝炎病毒感染方面，被证明比用非结构病毒蛋白C100进行酶联免疫吸附测定更有效。
