Kato N, Ikeda M, Mizutani T, Sugiyama K, Noguchi M, Hirohashi S, Shimotohno K
Virology Division, National Cancer Center Research Institute, Tokyo.
Jpn J Cancer Res. 1996 Aug;87(8):787-92. doi: 10.1111/j.1349-7006.1996.tb02101.x.
We established a replication system for hepatitis C virus (HCV) using the PH5CH non-neoplastic human hepatocyte line that had been immortalized with simian virus 40 large T antigen. In cells inoculated with sera derived from two HCV-positive blood donors, positive-stranded HCV RNA was detected up to 30 days postinoculation (p.i.). Semi-quantitative analysis of HCV RNA revealed that HCV multiplied during the period of culture. Sequence analysis of the HCV hypervariable region 1 (HVR1) in both cases indicated that HVR1 populations from the cells at 8 days p.i. were apparently different from those of the original inocula. HVR1 populations in infected cells became homogeneous or just a few species were selected over time. These results suggest that HCV is replicating in the human hepatocyte PH5CH cells. This culture system will be useful for detailed studies of the biological effects of HCV in human hepatocytes.
我们利用经猿猴病毒40大T抗原永生化的PH5CH非肿瘤性人肝细胞系建立了丙型肝炎病毒（HCV）复制系统。在用两名HCV阳性献血者的血清接种的细胞中，接种后30天内均检测到正链HCV RNA。HCV RNA的半定量分析显示，HCV在培养期间增殖。对这两例病例的HCV高变区1（HVR1）进行序列分析表明，接种后8天细胞中的HVR1群体明显不同于原始接种物。随着时间的推移，感染细胞中的HVR1群体变得同质化或仅选择了少数几种。这些结果表明HCV正在人肝细胞PH5CH细胞中复制。该培养系统将有助于详细研究HCV在人肝细胞中的生物学效应。
