由丙型肝炎病毒基因组5'区域编码的一种结构蛋白能有效检测病毒感染。
A structural protein encoded by the 5' region of the hepatitis C virus genome efficiently detects viral infection.
作者信息
Kato N, Hijikata M, Ootsuyama Y, Nakagawa M, Ohkoshi S, Shimotohno K
机构信息
Virology Division, National Cancer Center Research Institute, Tokyo.
出版信息
Jpn J Cancer Res. 1990 Nov;81(11):1092-4. doi: 10.1111/j.1349-7006.1990.tb02518.x.
Abstract
A structural region of the hepatitis C virus genome was molecularly cloned. A protein expressed in vitro by transcription followed by translation was precipitated immunologically by sera from patients with chronic hepatitis or hepatocellular carcinoma that were positive for antibody against the non-structural protein, C100, of hepatitis C virus, but not by sera from healthy persons. Thus, this structural protein should be useful for detection of infection with this virus.
摘要
丙型肝炎病毒基因组的一个结构区域被进行了分子克隆。通过转录后翻译在体外表达的一种蛋白质，能被慢性肝炎或肝细胞癌患者血清免疫沉淀，这些患者的血清中抗丙型肝炎病毒非结构蛋白C100抗体呈阳性，但健康人血清不能使其沉淀。因此，这种结构蛋白应有助于检测该病毒感染。
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1
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Proc Natl Acad Sci U S A. 1985 Jan;82(2):302-6. doi: 10.1073/pnas.82.2.302.
2
Prevalence of antibodies to hepatitis C virus in Italian patients with hepatocellular carcinoma.意大利肝细胞癌患者中丙型肝炎病毒抗体的流行情况。
Lancet. 1989 Oct 28;2(8670):1006-8. doi: 10.1016/s0140-6736(89)91016-7.
3
Prevalence of antibodies to hepatitis C virus in Spanish patients with hepatocellular carcinoma and hepatic cirrhosis.西班牙肝细胞癌和肝硬化患者丙型肝炎病毒抗体的流行情况。
Lancet. 1989 Oct 28;2(8670):1004-6. doi: 10.1016/s0140-6736(89)91015-5.
4
Isolation of a cDNA clone derived from a blood-borne non-A, non-B viral hepatitis genome.从经血传播的非甲非乙型病毒性肝炎基因组中分离出一个cDNA克隆。
Science. 1989 Apr 21;244(4902):359-62. doi: 10.1126/science.2523562.
5
An assay for circulating antibodies to a major etiologic virus of human non-A, non-B hepatitis.一种针对人类非甲非乙型肝炎主要致病病毒的循环抗体检测方法。
Science. 1989 Apr 21;244(4902):362-4. doi: 10.1126/science.2496467.
6
Detection of hepatitis C virus RNA in sera and liver tissues of non-A, non-B hepatitis patients using the polymerase chain reaction.运用聚合酶链反应检测非甲非乙型肝炎患者血清和肝组织中的丙型肝炎病毒RNA
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7
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Jpn J Exp Med. 1990 Jun;60(3):167-77.
8
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Jpn J Cancer Res. 1990 Jun-Jul;81(6-7):550-3. doi: 10.1111/j.1349-7006.1990.tb02605.x.
9
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