Sakai H, Siomi H, Shida H, Shibata R, Kiyomasu T, Adachi A
Laboratory of Gene Analysis, Kyoto University, Japan.
J Virol. 1990 Dec;64(12):5833-9. doi: 10.1128/JVI.64.12.5833-5839.1990.
The effect of rev-responsive element deletion on human immunodeficiency virus type 1 (HIV-1) and type 2 (HIV-2) gene expression was examined. The phenotypes of HIV-1 and HIV-2 provirus DNAs lacking the rev-responsive element, as determined by transfection experiments, were indistinguishable from those of virus DNAs carrying rev gene mutations. By using rev-response elements derived from these two viruses, we developed two monitoring systems to evaluate the functionality of HIV-1 rev, HIV-2 rev, and human T-lymphotropic virus type I rex. In both systems, HIV-1 rev and human T-lymphotropic virus type I rex transactivated HIV-2 very efficiently. On the contrary, HIV-2 rev and human T-lymphotropic virus type I rex were poor activators of HIV-1. No functional replacement of rex by HIV-2 rev was observed.
研究了Rev反应元件缺失对1型人类免疫缺陷病毒（HIV-1）和2型人类免疫缺陷病毒（HIV-2）基因表达的影响。通过转染实验确定，缺乏Rev反应元件的HIV-1和HIV-2前病毒DNA的表型与携带Rev基因突变的病毒DNA的表型没有区别。利用源自这两种病毒的Rev反应元件，我们开发了两个监测系统来评估HIV-1 Rev、HIV-2 Rev和1型人类嗜T淋巴细胞病毒Rex的功能。在这两个系统中，HIV-1 Rev和1型人类嗜T淋巴细胞病毒Rex都能非常有效地反式激活HIV-2。相反，HIV-2 Rev和1型人类嗜T淋巴细胞病毒Rex对HIV-1的激活作用较弱。未观察到HIV-2 Rev对Rex的功能替代。
