淋球菌porA假基因存在于广泛的淋病奈瑟菌菌株中的证据；作为诊断靶点的适用性。
Evidence that the gonococcal porA pseudogene is present in a broad range of Neisseria gonorrhoeae strains; suitability as a diagnostic target.
作者信息
Whiley David M, Anderson Trevor P, Barratt Kevin, Beaman Miles H, Buda Philip J, Carter Martine, Freeman Kevin, Hallsworth Peter, Limnios E Athena, Lum Gary, Merien Fabrice, Vernel-Pauillac Frédérique, Tapsall John W, Witt Michael J, Nissen Michael D, Sloots Theo P
机构信息
Microbiology Unit, Canterbury Health Laboratories, Christchurch, New Zealand.
出版信息
Pathology. 2006 Oct;38(5):445-8. doi: 10.1080/00313020600928253.
AIMS
The primary aim of the study was to determine if the gonococcal porA pseudogene is a stable sequence target for the detection of Neisseria gonorrhoeae by PCR.
METHODS
A total of 240 gonococcal strains from various geographic locations were tested by porA pseudogene PCR. In addition, porA pseudogene PCR positivity rates were compared with established gonococcal assays in three Australian states.
RESULTS
All N. gonorrhoeae isolates provided positive results in the porA pseudogene PCR. Positivity rates compared favourably with established gonococcal assays, with increased N. gonorrhoeae detection in the Northern Territory and Western Australia.
CONCLUSIONS
The results of this multicentre study provide further evidence that the porA pseudogene is highly conserved across a diverse range N. gonorrhoeae strains and is a suitable PCR target for routine detection of N. gonorrhoeae.
目的
本研究的主要目的是确定淋球菌porA假基因是否为通过聚合酶链反应（PCR）检测淋病奈瑟菌的稳定序列靶点。
方法
采用porA假基因PCR对来自不同地理位置的240株淋球菌菌株进行检测。此外，还在澳大利亚三个州将porA假基因PCR阳性率与既定的淋球菌检测方法进行了比较。
结果
所有淋病奈瑟菌分离株在porA假基因PCR中均呈阳性结果。与既定的淋球菌检测方法相比，阳性率良好，在北领地和西澳大利亚州淋病奈瑟菌的检测有所增加。
结论
这项多中心研究的结果提供了进一步的证据，表明porA假基因在多种淋病奈瑟菌菌株中高度保守，是常规检测淋病奈瑟菌的合适PCR靶点。
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