益生菌对豚鼠离体组织胃肠动力的影响。
Effects of probiotic bacteria on gastrointestinal motility in guinea-pig isolated tissue.
作者信息
Massi Matteo, Ioan Pierfranco, Budriesi Roberta, Chiarini Alberto, Vitali Beatrice, Lammers Karen M, Gionchetti Paolo, Campieri Massimo, Lembo Anthony, Brigidi Patrizia
机构信息
Department of Pharmaceutical Sciences, University of Bologna, Italy.
出版信息
World J Gastroenterol. 2006 Oct 7;12(37):5987-94. doi: 10.3748/wjg.v12.i37.5987.
AIM
To evaluate the intestinal motility changes evoked by 8 bacterial strains belonging to Bifidobacterium, Lactobacillus and Streptococcus genera within the probiotic preparation VSL#3.
METHODS
Ileum and proximal colon segments isolated from guinea-pigs were used as a study model. Entire cells and cell fractions (cell debris, cell wall fraction, cytoplasmatic fraction, proteinaceous and non-proteinaceous cytoplasmatic components) of VSL#3 strains and, as controls, Escherichia coli, Salmonella aboni and Bacillus licheniformis were tested in this in vitro model.
RESULTS
Among the bacterial cell fractions tested, only the cytoplasmatic fraction modified intestinal motility. Lactobacillus strains stimulated the contraction of ileum segment, whereas all probiotic strains tested induced proximal colon relaxation response. The non-proteinaceous cytoplasmatic components were responsible for the colon relaxation.
CONCLUSION
The results obtained in this study suggest that the proximal colon relaxation activity showed by the probiotic bacteria could be one of the possible mechanisms of action by which probiotics exert their positive effects in regulating intestinal motility.
目的
评估益生菌制剂VSL#3中属于双歧杆菌属、乳酸杆菌属和链球菌属的8种细菌菌株引起的肠道动力变化。
方法
从豚鼠分离的回肠和近端结肠段用作研究模型。在该体外模型中测试了VSL#3菌株的全细胞和细胞组分（细胞碎片、细胞壁组分、细胞质组分、蛋白质和非蛋白质细胞质成分），并以大肠杆菌、阿博尼沙门氏菌和地衣芽孢杆菌作为对照。
结果
在所测试的细菌细胞组分中，只有细胞质组分改变了肠道动力。乳酸杆菌菌株刺激回肠段收缩，而所有测试的益生菌菌株均诱导近端结肠松弛反应。非蛋白质细胞质成分是导致结肠松弛的原因。
结论
本研究获得的结果表明，益生菌显示出的近端结肠松弛活性可能是益生菌在调节肠道动力中发挥积极作用的可能作用机制之一。
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