Agence Française de Sécurité Sanitaire des Aliments (AFSSA), Unité Bactériologie Bovine et Hygiène des Viandes, Lyon, France.
Foodborne Pathog Dis. 2009 Nov;6(9):1107-11. doi: 10.1089/fpd.2009.0303.
The goal of this study was to assess the presence of enterococci species presenting van-mediated glycopeptide resistance in French cattle. Fecal samples were collected from healthy and sick animals, and enterococci were screened for vancomycin resistance. Vancomycin resistance was principally encountered in Enterococcus gallinarum and Enterococcus casseliflavus strains. However, glycopeptide resistance was detected in three different species of enterococci (E. faecalis, E. faecium, and E. casseliflavus). Molecular characterization of the genetic support proved that they all presented the prototypic VanA element. Interestingly, the E. casseliflavus strain displayed a remarkable VanB phenotype/vanA-vanC genotype. Transferability, associated resistances, and factors of vanA cotransfer were sought. This study proved that acquired vanA genes can still be detected in food-producing animals more than a decade after the avoparcin ban. Indeed, calves, which are recurrently exposed to antibiotics in France, may allow the re-emergence of glycopeptide resistance through coselection factors, and this might potentially be concerning for human health.
本研究旨在评估法国牛群中存在的具有 van 介导的糖肽类耐药性的肠球菌属物种。采集了健康和患病动物的粪便样本，并对肠球菌进行了万古霉素耐药性筛选。万古霉素耐药性主要见于鸡肠球菌和黄色肠球菌菌株。然而，在三种不同的肠球菌（粪肠球菌、屎肠球菌和黄色肠球菌）中检测到了糖肽类耐药性。遗传支持的分子特征证明它们均具有典型的 VanA 元件。有趣的是，黄色肠球菌菌株表现出显著的 VanB 表型/vanA-vanC 基因型。还研究了可转移性、相关耐药性以及 vanA 共转移因子。本研究证明，在阿伏帕星禁令实施十多年后，仍可在食用动物中检测到获得性的 vanA 基因。事实上，法国的小牛经常接触抗生素，可能会通过共选择因素重新出现糖肽类耐药性，这可能对人类健康构成潜在威胁。
