Teeple E, Fleming B C, Mechrefe A P, Crisco J J, Brady M F, Jay G D
Bioengineering Laboratory, Department of Orthopaedics, Brown Medical School/Rhode Island Hospital, Providence, RI 02903, USA.
Osteoarthritis Cartilage. 2007 Mar;15(3):309-15. doi: 10.1016/j.joca.2006.08.011. Epub 2006 Sep 28.
To apply a pendulum technique to detect changes in the coefficient of friction of the articular cartilage of the intact guinea pig tibiofemoral joint after proteolytic disruption.
Twenty-two hind limbs were obtained from 11 3-month old Hartley guinea pigs. Twenty knees were block-randomized to one of two treatment groups receiving injections of: (1) alpha-chymotrypsin (to disrupt the superficial layer of the articular surface) or (2) saline (sham; to control for the effects of the intra-articular injection). The legs were mounted in a pendulum where the knee served as the fulcrum. The decay in pendulum amplitude as a function of oscillation number was first recorded and the coefficient of friction of the joint was determined from these data before injection. Ten microliters of either isotonic saline or 1 Unit/microL alpha-chymotrypsin was then injected into the intra-articular joint space and incubated for 2h. The pendulum test was repeated. Changes in the coefficient of friction between the sham and alpha-chymotrypsin joints were compared. One additional pair of knees was used for histological study of the effects of the injections.
Treatment with alpha-chymotrypsin significantly increased the coefficient of friction of the guinea pig knee by 74% while sham treatment decreased it by 8%. Histological sections using Gomori trichrome stain verified that the lamina splendens was damaged following treatment with alpha-chymotrypsin and not following saline treatment.
Treatment with alpha-chymotrypsin induces mild cartilage surface damage and increases the coefficient of friction in the Hartley guinea pig knee.
应用摆锤技术检测蛋白水解破坏后完整豚鼠胫股关节软骨摩擦系数的变化。
从11只3月龄的Hartley豚鼠获取22条后肢。将20个膝关节进行区组随机分组，分为两个治疗组，分别接受以下注射：（1）α-糜蛋白酶（破坏关节表面浅层）或（2）生理盐水（假手术；用于控制关节内注射的影响）。将腿部安装在以膝关节为支点的摆锤上。首先记录摆锤振幅随振荡次数的衰减情况，并根据注射前这些数据确定关节的摩擦系数。然后将10微升等渗盐水或1单位/微升α-糜蛋白酶注入关节腔内，并孵育2小时。重复摆锤试验。比较假手术组和α-糜蛋白酶组关节摩擦系数的变化。另外一对膝关节用于注射效果的组织学研究。
α-糜蛋白酶治疗使豚鼠膝关节的摩擦系数显著增加74%，而假手术治疗使其降低8%。使用Gomori三色染色的组织学切片证实，α-糜蛋白酶治疗后亮层受损，而生理盐水治疗后未受损。
α-糜蛋白酶治疗会导致Hartley豚鼠膝关节软骨表面轻度损伤并增加摩擦系数。
