Morini Luca, Politi Lucia, Zucchella Alessandra, Polettini Aldo
Department of Legal Medicine and Public Health, University of Pavia, Via Forlanini 12-27100 Pavia, Italy.
Clin Chim Acta. 2007 Feb;376(1-2):213-9. doi: 10.1016/j.cca.2006.08.024. Epub 2006 Sep 29.
Ethyl glucuronide (EtG) and ethyl sulphate (EtS) are two ethanol metabolites that can be detected in serum up to 8 h after ethanol elimination. Their presence is therefore indicative of recent ethanol consumption in case of delayed sampling after an event (e.g. car crash).
A LC-ESI-MS-MS method for the determination of EtG and EtS in serum was developed and validated. Two product ions together with the parent ions were monitored for identification. Pentadeuterated-EtG was used as internal standard.
Excellent linearity for EtG (from 0.22 to 45 micromol/l) and EtS (from 0.40 to 80 micromol/l) was observed (r(2)>or=0.9998). LOD and LLOQ were 0.04 and 0.20 micromol/l for EtG and 0.08 and 0.40 micromol/l for EtS, respectively. Accuracy (bias) and precision (relative standard deviation), studied at four different quality control levels, were always better than 7%. Matrix effects were found to be negligible. The method was applied to several samples obtained from known alcoholics and social drinkers.
A sensitive and specific determination of EtG and EtS in serum samples was achieved despite a simple and fast sample preparation. To our knowledge, this is the first fully validated method for the simultaneous determination of the two alcohol metabolites in serum.
葡萄糖醛酸乙酯（EtG）和硫酸乙酯（EtS）是两种乙醇代谢产物，在乙醇消除后长达8小时可在血清中检测到。因此，在事件发生后延迟采样（如车祸）的情况下，它们的存在表明近期有乙醇摄入。
建立并验证了一种用于测定血清中EtG和EtS的液相色谱 - 电喷雾串联质谱法（LC - ESI - MS - MS）。监测两个产物离子以及母离子用于鉴定。使用五氘代 - EtG作为内标。
观察到EtG（0.22至45微摩尔/升）和EtS（0.40至80微摩尔/升）具有出色的线性（r²≥0.9998）。EtG的检测限（LOD）和定量下限（LLOQ）分别为0.04和0.20微摩尔/升，EtS的检测限和定量下限分别为0.08和0.40微摩尔/升。在四个不同质量控制水平下研究的准确度（偏差）和精密度（相对标准偏差）始终优于7%。发现基质效应可忽略不计。该方法应用于从已知酗酒者和社交饮酒者获得的多个样本。
尽管样品制备简单快速，但仍实现了血清样本中EtG和EtS的灵敏且特异的测定。据我们所知，这是首次同时测定血清中两种乙醇代谢产物的完全验证方法。
