Einen Jørn, Øvreås Lise
Department of Biology, University of Bergen, Jahnebakken 5, 5020 Bergen, Norway.
Int J Syst Evol Microbiol. 2006 Oct;56(Pt 10):2455-2461. doi: 10.1099/ijs.0.64404-0.
Four yellow-pigmented, Gram-negative, motile strains were isolated from the glassy rind of submarine basaltic lava from the Jan Mayen area of the Norwegian/Greenland Sea at a depth of 1300 m below sea level. The four strains had identical 16S rRNA gene sequences and were indistinguishable in all phenotypic and chemotypic tests performed, indicating that they belonged to the same species. The strains had an obligately aerobic chemo-organotrophic metabolism. The strains were capable of growth at temperatures between -2 and 34 degrees C, at pH between 6.5 and 8.6, and at sea salt concentrations between 3 and 60 g l(-1). The strains were able to utilize organic acids, amino acids and sugars but not alcohols; they were also capable of hydrolysing a wide range of macromolecules. The predominant fatty acids were 15 : 0 iso, 15 : 1 iso, 15 : 0 iso 3-OH and 17 : 0 iso 3-OH. The mean DNA G+C content of the strains was 31.4 mol%. 16S rRNA gene sequence analysis indicated that the strains were affiliated to the genera Gaetbulibacter and Algibacter. However, phenotypic characteristics, especially aerobic metabolism, suggested that the strains should be placed within a new genus. On the basis of the polyphasic characterization of the four strains, it is suggested that the strains be included in the family Flavobacteriaceae as representatives of a novel species in a new genus, for which the name Flaviramulus basaltis gen. nov., sp. nov. is proposed. The type strain is H35(T) (=CIP 109091(T)=DSM 18180(T)).
从挪威海/格陵兰海扬马延岛海域海平面以下1300米深处的海底玄武岩熔岩的玻璃状外皮中分离出四株黄色色素沉着、革兰氏阴性、具运动性的菌株。这四株菌株具有相同的16S rRNA基因序列，并且在所有进行的表型和化学型测试中均无法区分，表明它们属于同一物种。这些菌株具有专性需氧的化学有机营养代谢。这些菌株能够在-2至34摄氏度、pH值在6.5至8.6之间以及海盐浓度在3至60克/升的条件下生长。这些菌株能够利用有机酸、氨基酸和糖类，但不能利用醇类；它们还能够水解多种大分子。主要脂肪酸为15:0异戊酸、15:1异戊酸、15:0异戊酸3-OH和17:0异戊酸3-OH。这些菌株的平均DNA G+C含量为31.4摩尔%。16S rRNA基因序列分析表明这些菌株隶属于盖特布利杆菌属和藻杆菌属。然而，表型特征，尤其是需氧代谢，表明这些菌株应归入一个新属。基于对这四株菌株的多相特征分析，建议将这些菌株作为一个新属中一个新物种的代表归入黄杆菌科，为此提出新属名Flaviramulus basaltis gen. nov., sp. nov.。模式菌株为H35(T) (=CIP 109091(T)=DSM 18180(T))。
