Wang Ning, Xu Fei, Zhang Xi-Ying, Chen Xiu-Lan, Qin Qi-Long, Zhou Bai-Cheng, Zhang Yu-Zhong, Shi Mei
State Key Laboratory of Microbial Technology, Shandong University, Jinan 250100, PR China.
Marine Biotechnology Research Center, Shandong University, Jinan 250100, PR China.
Int J Syst Evol Microbiol. 2017 Dec;67(12):5187-5192. doi: 10.1099/ijsem.0.002439. Epub 2017 Oct 25.
A Gram-negative, aerobic, yellow pigmented, non-flagellated, non-gliding, rod-shaped bacterial strain, designated SM1355, was isolated from Antarctic intertidal sediment collected near the Chinese Antarctic Great Wall Station. The strain grew at 4-35 °C and with 0.5-7.0 % (w/v) NaCl. It hydrolysed aesculin but didn't reduce nitrate to nitrite. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain SM1355 formed a distinct phylogenetic lineage within the family Flavobacteriaceae, sharing the highest 16S rRNA gene sequence similarity with Flaviramulus ichthyoenteri (96.3 %) and fairly high sequence similarities (95.0-96.0 %) with over 20 recognized species in eight genera of the family Flavobacteriaceae. The predominant fatty acids were anteiso-C15 : 0, iso-C15 : 0 and iso-C15 : 1 G. The major polar lipids were phosphatidylethanolamine and one unidentified lipid. The genomic DNA G+C content of strain SM1355 was 36.2 mol%. Based on the results of the polyphasic characterization for strain SM1355, it is identified as the representative of a novel species in a new genus of the family Flavobacteriaceae, for which the name Changchengzhania lutea gen. nov., sp. nov. is proposed. The type strain of Changchengzhania lutea is SM1355 (=JCM 30336=CCTCC AB 2014246).
从中国南极长城站附近采集的南极潮间带沉积物中分离出一株革兰氏阴性、需氧、产黄色色素、无鞭毛、不滑动的杆状细菌，命名为SM1355。该菌株在4-35 °C和0.5-7.0 %（w/v）NaCl条件下生长。它能水解七叶苷，但不能将硝酸盐还原为亚硝酸盐。基于16S rRNA基因序列的系统发育分析表明，菌株SM1355在黄杆菌科内形成了一个独特的系统发育谱系，与鱼肠黄杆菌（Flaviramulus ichthyoenteri）的16S rRNA基因序列相似度最高（96.3 %），与黄杆菌科八个属的20多个已确认物种的序列相似度也相当高（95.0-96.0 %）。主要脂肪酸为anteiso-C15 : 0、iso-C15 : 0和iso-C15 : 1 G。主要极性脂为磷脂酰乙醇胺和一种未鉴定的脂质。菌株SM1355的基因组DNA G+C含量为36.2 mol%。基于对菌株SM1355的多相分类学特征分析结果，它被鉴定为黄杆菌科一个新属中一个新物种的代表，为此提出新属名长城菌属（Changchengzhania）、新种名黄色长城菌（Changchengzhania lutea）。黄色长城菌的模式菌株为SM1355（=JCM 30336=CCTCC AB 2014246）。
