Gough D B, Boyle B, Joyce W P, Delaney C P, McGeeney K F, Gorey T F, Fitzpatrick J M
Department of Surgery, Mater Misericordiae Hospital and University College Dublin, Ireland.
Br J Surg. 1990 Nov;77(11):1256-9. doi: 10.1002/bjs.1800771119.
Oxygen free radical activity and inhibition were examined in experimental pancreatitis. Twenty-five rats were randomized to five groups: controls received intravenous saline, to simulate pancreatitis one group received intravenous caerulein (5 micrograms kg-1 h-1), and three groups received sodium taurocholate via the pancreatic duct (0.2 ml, 5 per cent), either alone, following allopurinol or immediately before superoxide dismutase. Chemiluminescence (a phenomenon based on the emission of light during chemical reactions and which is dependent on oxygen free radical activity) was used as an index of oxygen free radical activity and was measured in tissue samples at 5-min intervals following induction of pancreatitis. The control mean(s.e.m.) serum amylase level 1 h after induction of pancreatitis was 635(13) units. It was significantly elevated in caerulein-induced pancreatitis, 1833(118) units (P less than 0.05) and exceeded 3000 units in all taurocholate-infused animals. Mean(s.e.m.) chemiluminescence ranged from 44 (8) mV 100 mg-1 at time zero to 404(113) mV 100 mg-1 at 1 h in controls. In caerulein-induced pancreatitis mean(s.e.m.) chemiluminescence peaked at 20 min (1399(239) mV 100 mg-1, P less than 0.02) and in taurocholate-induced pancreatitis at 15 min (2316(95) mV 100 mg-1, P less than 0.004). Superoxide dismutase significantly reduced chemiluminescence and hyperamylasaemia in taurocholate groups. Increasing oxygen free radical activity paralleled evolving pancreatitis. Superoxide dismutase may have a therapeutic role in pancreatitis.
在实验性胰腺炎中检测了氧自由基活性及抑制情况。25只大鼠被随机分为五组：对照组静脉注射生理盐水，为模拟胰腺炎，一组静脉注射蛙皮素（5微克/千克·小时），另外三组通过胰管注射牛磺胆酸钠（0.2毫升，5%），分别单独注射、在注射别嘌呤醇后注射或在超氧化物歧化酶注射前立即注射。化学发光（一种基于化学反应过程中发光且依赖于氧自由基活性的现象）被用作氧自由基活性的指标，并在诱导胰腺炎后每隔5分钟在组织样本中进行测量。诱导胰腺炎1小时后，对照组血清淀粉酶水平平均值（标准误）为635（13）单位。在蛙皮素诱导的胰腺炎中显著升高，达1833（118）单位（P<0.05），在所有注射牛磺胆酸钠的动物中超过3000单位。对照组化学发光平均值（标准误）从零时刻的44（8）毫伏/100毫克在1小时时升至404（113）毫伏/100毫克。在蛙皮素诱导的胰腺炎中，化学发光平均值（标准误）在20分钟时达到峰值（1399（239）毫伏/100毫克，P<0.02），在牛磺胆酸钠诱导的胰腺炎中在15分钟时达到峰值（2316（95）毫伏/100毫克，P<0.004）。超氧化物歧化酶显著降低了牛磺胆酸钠组的化学发光及高淀粉酶血症。氧自由基活性增加与胰腺炎进展平行。超氧化物歧化酶可能在胰腺炎治疗中发挥作用。
