对吗啡条件性奖赏效应的敏化调节大鼠海马体中的基因表达。
Sensitization to the conditioned rewarding effects of morphine modulates gene expression in rat hippocampus.
作者信息
Marie-Claire Cynthia, Courtin Cindie, Robert Amelie, Gidrol Xavier, Roques Bernard P, Noble Florence
机构信息
CNRS, UMR7157, INSERM U705, Université Paris Descartes, Neuropsychopharmacologie des Addictions, 4 avenue de l'Observatoire, F-75006 Paris, France.
出版信息
Neuropharmacology. 2007 Feb;52(2):430-5. doi: 10.1016/j.neuropharm.2006.08.012. Epub 2006 Oct 2.
Opiates addiction is characterized by its long-term persistence. In order to study the enduring changes in long-term memory in hippocampus, a pivotal region for this process, we used suppression subtractive hybridization to compare hippocampal gene expression in morphine and saline-treated rats. Animals were subjected to an extended place preference paradigm consisting of four conditioning phases. Sensitization to the reinforcing effects of the drug occurred after three conditioning phases. After 25 days of treatment rats were euthanized and the complementary DNA (cDNA) from the hippocampus of morphine-dependent and saline-treated animals were then screened for differentially expressed cDNAs. The selected 177 clones were then subjected to a microarray procedure and 20 clones were found differentially regulated. The pattern of regulated genes suggests impairments in neurotransmitter release and the activation of neuroprotective pathways.
阿片类药物成瘾的特点是具有长期持续性。为了研究海马体（这一过程的关键区域）长期记忆中的持久变化，我们使用抑制性消减杂交技术比较吗啡处理组和生理盐水处理组大鼠海马体中的基因表达。动物接受了一个由四个条件阶段组成的扩展位置偏好范式。经过三个条件阶段后，动物对药物强化作用产生了敏化。治疗25天后，处死大鼠，然后筛选吗啡依赖动物和生理盐水处理动物海马体中的互补DNA（cDNA），以寻找差异表达的cDNA。然后对所选的177个克隆进行微阵列分析，发现有20个克隆的表达存在差异调节。基因调控模式表明神经递质释放受损以及神经保护途径被激活。
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