Pirruccello S J, Lang M S
Department of Pathology and Microbiology, University of Nebraska Medical Center, Omaha 68105-1065.
Blood. 1990 Dec 1;76(11):2343-7.
In the hematopoietic system, the B-cell associated antigen CD24 is expressed at high density on B cells, B-cell precursors, and B-cell malignancies as well as at low density on peripheral blood polymorphonuclear leukocytes. The 42-Kd sialoglycoprotein has not been previously demonstrated to be expressed on T cells, thymocytes, or T-cell malignancies. We identified three patients with mycosis fungoides/Sézary syndrome that showed low density expression of the CD24-related epitope recognized by antibody BA-1 on circulating T cells. All three patients had Sézary cells by morphologic assessment and clonal T-cell populations in the peripheral blood by gene rearrangement studies. In two of these patients, indirect immunofluorescence with a panel of six anti-CD24 monoclonal antibodies demonstrated reactivity for two of six antibodies in one case and only one of six antibodies in the other. The biologic significance of CD24-related epitope expression on circulating T cells in mycosis fungoides/Sézary syndrome is unclear. However, these findings suggest that differential, low density expression of CD24-related epitopes (BA-1+, OKB2-) may be a useful phenotypic marker for identifying circulating Sézary cells.
在造血系统中，B细胞相关抗原CD24在B细胞、B细胞前体和B细胞恶性肿瘤上高密度表达，在外周血多形核白细胞上低密度表达。此前尚未证实这种42千道尔顿的唾液糖蛋白在T细胞、胸腺细胞或T细胞恶性肿瘤上表达。我们鉴定出3例蕈样肉芽肿/塞扎里综合征患者，其循环T细胞上显示出抗体BA-1识别的CD24相关表位的低密度表达。通过形态学评估，所有3例患者均有塞扎里细胞，通过基因重排研究发现外周血中有克隆性T细胞群体。在其中2例患者中，用一组6种抗CD24单克隆抗体进行间接免疫荧光检测，结果显示，1例患者对6种抗体中的2种有反应，另1例患者仅对6种抗体中的1种有反应。蕈样肉芽肿/塞扎里综合征循环T细胞上CD24相关表位表达的生物学意义尚不清楚。然而，这些发现表明，CD24相关表位（BA-1+，OKB2-）的差异性低密度表达可能是识别循环塞扎里细胞的一个有用的表型标志物。
