Ngo Kiet A, Maffei Joseph G, Dupuis Alan P, Kauffman Elizabeth B, Backenson P Bryon, Kramer Laura D
The Arbovirus Laboratories, Wadsworth Center, New York State Department of Health, 5668 State Farm Rd. Slingerlands, NY 12159, USA.
J Med Entomol. 2006 Sep;43(5):1004-9. doi: 10.1603/0022-2585(2006)43[1004:iobsvb]2.0.co;2.
During routine arbovirus surveillance from 2000 to 2004 in New York state (NYS), 14,788 mosquito pools making up 36 species and nine genera were inoculated onto Vero cell cultures to test for abroad spectrum of viruses. Forty-six percent of viruses isolated in cell culture from species, excluding Culex pipiens L. and Culex restuans Theobald, were identified as Bunyamwera serogroup viruses. Here, we report the distribution and level of Bunyamwera activity in NYS detected during this period. We developed specific primers for Cache Valley virus (family Bunyaviridae, genus Orthobunyavirus, CVV) and Potosi virus (family Bunyaviridae, genus Orthobunyavirus, POTV), to facilitate rapid molecular identification of these viruses. Viral RNA was detected in 12 mosquito species by reverse transcription-polymerase chain reaction, with the majority isolated from Aedes trivittatus (Coquillet). We report the first POTV isolation in NYS and describe the development of specific primers to identify both POTV and CVV.
2000年至2004年在纽约州（NYS）进行常规虫媒病毒监测期间，将由36个物种和9个属组成的14788个蚊群接种到Vero细胞培养物中，以检测多种病毒。从细胞培养物中分离出的病毒（不包括致倦库蚊和环跗库蚊）中，46%被鉴定为布尼亚姆韦拉血清群病毒。在此，我们报告在此期间在纽约州检测到的布尼亚姆韦拉病毒的分布和活性水平。我们开发了针对卡奇谷病毒（布尼亚病毒科，正布尼亚病毒属，CVV）和波托西病毒（布尼亚病毒科，正布尼亚病毒属，POTV）的特异性引物，以促进对这些病毒的快速分子鉴定。通过逆转录-聚合酶链反应在12种蚊虫中检测到病毒RNA，其中大多数分离自三带喙库蚊。我们报告了纽约州首次分离出POTV，并描述了用于鉴定POTV和CVV的特异性引物的开发。
