Meyers Matthew T, Bahnson Charlie S, Hanlon Michael, Kopral Christine, Srisinlapaudom Saengchan, Cochrane Zachary N, Sabas Carlene E, Saiyasombat Rungrat, Burrough Eric R, Plummer Paul J, O'Connor Annette M, Marshall Katherine L, Blitvich Bradley J
1 Department of Veterinary Microbiology and Preventive Medicine, College of Veterinary Medicine, Iowa State University , Ames, Iowa.
2 United States Department of Agriculture, Animal and Plant Health Inspection Service, Veterinary Services, Center for Epidemiology and Animal Health , Fort Collins, Colorado.
Vector Borne Zoonotic Dis. 2015 Nov;15(11):683-93. doi: 10.1089/vbz.2015.1810.
A cross-sectional study was performed to identify operation-level risk factors associated with prevalence of antibody to Bunyamwera (BUN) serogroup viruses in sheep in the United States. Sera were obtained from 5150 sheep in 270 operations located in 22 states (three in the west, nine central states, and 10 in the east) and tested at a dilution of 1:20 by a plaque reduction neutralization test (PRNT) using Cache Valley virus (CVV). Antibodies that neutralized CVV were identified in 1455 (28%) sheep. Animal-level seroprevalence was higher in the east (49%) than the central (17%) and western (10%) states. A convenient subset (n = 509) of sera with antibodies that neutralized CVV was titrated and further analyzed by PRNT using all six BUN serogroup viruses that occur in the United States: CVV, Lokern virus (LOKV), Main Drain virus (MDV), Northway virus (NORV), Potosi virus (POTV), and Tensaw virus (TENV). Antibodies to CVV and LOKV were identified in sheep in all three geographic regions; MDV and POTV activity was detected in the central and eastern states, NORV activity was restricted to the west, and antibodies to TENV were not detected in any sheep. Several management factors were significantly associated with the presence of antibodies to BUN serogroup viruses. For instance, sheep housed during the lambing season inside structures that contained four walls and a roof and a door closed most of the time were more likely to be seropositive than other sheep. In contrast, herded/open-range sheep were less likely to be seropositive than their counterparts. These data can be used by producers to implement strategies to reduce the likelihood of BUN serogroup virus infection and improve the health and management practices of sheep.
开展了一项横断面研究，以确定与美国绵羊中布尼亚姆韦拉（BUN）血清群病毒抗体流行率相关的操作层面风险因素。从位于22个州（西部3个、中部9个州和东部10个州）的270个养殖场的5150只绵羊中采集血清，并使用卡奇谷病毒（CVV）通过蚀斑减少中和试验（PRNT）以1:20的稀释度进行检测。在1455只（28%）绵羊中鉴定出中和CVV的抗体。东部地区（49%）的动物血清阳性率高于中部（17%）和西部（10%）各州。对一个方便选取的中和CVV抗体血清子集（n = 509）进行滴定，并使用在美国出现的所有六种BUN血清群病毒通过PRNT进一步分析：CVV、洛肯病毒（LOKV）、主排水病毒（MDV）、诺斯韦病毒（NORV）、波托西病毒（POTV）和滕索病毒（TENV）。在所有三个地理区域的绵羊中均鉴定出针对CVV和LOKV的抗体；在中部和东部各州检测到MDV和POTV活性，NORV活性仅限于西部，且在任何绵羊中均未检测到针对TENV的抗体。几个管理因素与BUN血清群病毒抗体的存在显著相关。例如，在产羔季节期间饲养在有四面墙、一个屋顶且大部分时间门关闭的建筑物内的绵羊比其他绵羊更有可能血清呈阳性。相比之下，放牧/开放式放牧的绵羊比圈养绵羊血清呈阳性的可能性更小。生产者可利用这些数据实施策略，以降低BUN血清群病毒感染的可能性，并改善绵羊的健康和管理措施。
