用于人类疫苗接种的候选巨细胞病毒毒株。
Candidate cytomegalovirus strain for human vaccination.
作者信息
Plotkin S A, Furukawa T, Zygraich N, Huygelen C
出版信息
Infect Immun. 1975 Sep;12(3):521-7. doi: 10.1128/iai.12.3.521-527.1975.
Abstract
A strain of human cytomegalovirus called Towne was isolated in WI-38 human fibrolast cell cultures from the urine of an infected infant. It was then passaged 125 times in WI-38, including three clonings, and a pool was prepared in the same cell substrate for use as a potential live attenuated vaccine. The Towne virus has a broad antigenicity and cross-reacts with the AD-169 strain. Several markers of the Towne virus were found which differentiated it from fresh isolates. One of these was resistance of the former to trypsin. The Towne virus was tested for freedom from oncogenicity or other harmful effects in preparation for tests in humans.
摘要
一种名为汤氏（Towne）的人巨细胞病毒株，是从一名受感染婴儿的尿液中，在WI-38人成纤维细胞培养物中分离出来的。然后它在WI-38细胞中传代125次，包括三次克隆，并在相同的细胞基质中制备了一批病毒，用作潜在的减毒活疫苗。汤氏病毒具有广泛的抗原性，并且与AD-169毒株有交叉反应。发现了汤氏病毒的几个特征，这些特征使其与新鲜分离株有所区别。其中之一是前者对胰蛋白酶具有抗性。为准备在人体中进行试验，对汤氏病毒进行了无致癌性或其他有害影响的检测。
相似文献
1
Candidate cytomegalovirus strain for human vaccination.用于人类疫苗接种的候选巨细胞病毒毒株。
Infect Immun. 1975 Sep;12(3):521-7. doi: 10.1128/iai.12.3.521-527.1975.
2
Immunisation trials with live attenuated cytomegalovirus TOWNE 125.减毒活巨细胞病毒TOWNE 125免疫试验。
Infection. 1975;3(2):111-4. doi: 10.1007/BF01641052.
3
Cytomegalovirus vaccine prepared in WI-38.
Dev Biol Stand. 1976;37:301-5.
4
[Further observations on the adaptation to human cells, of a strain of cytomegalic virus from the field-mouse (Apodemus sylvaticus)].[对来自田鼠（森林姬鼠）的一株巨细胞病毒适应人类细胞的进一步观察]
C R Acad Hebd Seances Acad Sci D. 1972 May 24;274(21):2920-3.
5
Ultrastructural and biological properties of a cytomegalovirus rescued from a human paraganglioma.
Cancer Res. 1971 May;31(5):542-9.
6
Comparative virulence and immunogenicity of the Towne strain and a nonattenuated strain of cytomegalovirus.巨细胞病毒汤氏株与一株未减毒毒株的相对毒力和免疫原性
Ann Intern Med. 1984 Oct;101(4):478-83. doi: 10.7326/0003-4819-101-4-478.
7
Persistent shedding of cytomegalovirus in the urine of healthy Rhesus monkeys.
Proc Soc Exp Biol Med. 1974 Mar;145(3):794-801. doi: 10.3181/00379727-145-37897.
8
Humoral immune response to cytomegalovirus Towne vaccine strain and to Toledo low-passage strain.
J Infect Dis. 1989 May;159(5):851-9. doi: 10.1093/infdis/159.5.851.
9
Vaccination of pediatric nurses with live attenuated cytomegalovirus.
Am J Dis Child. 1982 Apr;136(4):294-6. doi: 10.1001/archpedi.1982.03970400012003.
10
Prevention of cytomegalovirus disease by Towne strain live attenuated vaccine.托恩株减毒活疫苗预防巨细胞病毒病
Birth Defects Orig Artic Ser. 1984;20(1):271-87.
引用本文的文献
1
Replication-deficient whole-virus vaccines against cytomegalovirus induce protective immunity in a guinea pig congenital infection model.针对巨细胞病毒的复制缺陷型全病毒疫苗在豚鼠先天性感染模型中诱导保护性免疫。
J Virol. 2025 Jul 22;99(7):e0020725. doi: 10.1128/jvi.00207-25. Epub 2025 Jun 11.
2
Developing a Vaccine Against Human Cytomegalovirus: Identifying and Targeting HCMV's Immunological Achilles' Heel.开发针对人类巨细胞病毒的疫苗：识别并靶向人巨细胞病毒的免疫弱点。
Vaccines (Basel). 2025 Apr 22;13(5):435. doi: 10.3390/vaccines13050435.
3
Exploring the Potential of Cytomegalovirus-Based Vectors: A Review.探索巨细胞病毒载体的潜力：综述。
Viruses. 2023 Oct 2;15(10):2043. doi: 10.3390/v15102043.
4
Congenital Cytomegalovirus and Hearing Loss: The State of the Art.先天性巨细胞病毒与听力损失：最新进展
J Clin Med. 2023 Jul 3;12(13):4465. doi: 10.3390/jcm12134465.
5
An Update on Current Antiviral Strategies to Combat Human Cytomegalovirus Infection.当前抗人巨细胞病毒感染的抗病毒策略更新。
Viruses. 2023 Jun 12;15(6):1358. doi: 10.3390/v15061358.
6
The RL13 Temperance Factor Represses Replication of the Highly Cell Culture-Adapted Towne Strain of Human Cytomegalovirus.RL13 抑素因子抑制高度适应细胞培养的人类巨细胞病毒 Towne 株的复制。
Viruses. 2023 Apr 21;15(4):1023. doi: 10.3390/v15041023.
7
Envelope Glycoprotein based multi-epitope vaccine against a co-infection of Human Herpesvirus 5 and Human Herpesvirus 6 using in silico strategies.基于包膜糖蛋白的多表位疫苗，通过计算机模拟策略针对人类疱疹病毒 5 和人类疱疹病毒 6 的合并感染。
Glycoconj J. 2022 Dec;39(6):711-724. doi: 10.1007/s10719-022-10083-7. Epub 2022 Oct 13.
8
G/S Cell Cycle Induction by Epstein-Barr Virus BORF2 Is Mediated by P53 and APOBEC3B.EBV BORF2 通过 P53 和 APOBEC3B 诱导细胞周期
J Virol. 2022 Sep 28;96(18):e0066022. doi: 10.1128/jvi.00660-22. Epub 2022 Sep 7.
9
Antibodies to combat viral infections: development strategies and progress.针对病毒感染的抗体：开发策略与进展。
Nat Rev Drug Discov. 2022 Sep;21(9):676-696. doi: 10.1038/s41573-022-00495-3. Epub 2022 Jun 20.
10
Common Polymorphisms in the Glycoproteins of Human Cytomegalovirus and Associated Strain-Specific Immunity.人巨细胞病毒糖蛋白的常见多态性及其与株特异性免疫的关系。
Viruses. 2021 Jun 9;13(6):1106. doi: 10.3390/v13061106.
本文引用的文献
1
Virulence and attenuation of murine cytomegalovirus.鼠巨细胞病毒的毒力和减毒。
Infect Immun. 1971 Feb;3(2):228-36. doi: 10.1128/iai.3.2.228-236.1971.
2
Post-transfusion cytomegaloviremia and persistence of cytomegalovirus in blood.输血后巨细胞病毒血症和巨细胞病毒在血液中的持续存在。
Infect Immun. 1971 Jan;3(1):159-63. doi: 10.1128/iai.3.1.159-163.1971.
3
Application of a microtechnique to viral serological investigations.一种微技术在病毒血清学研究中的应用。
J Immunol. 1962 Mar;88:320-9.
4
Postperfusion syndrome. A review and report of 21 cases.灌注后综合征。21例病例回顾与报告
Am Heart J. 1966 Jul;72(1):116-23. doi: 10.1016/0002-8703(66)90634-x.
5
The attenuation, with loss of oncogenicity, of the herpes-type virus of Marek's disease (strain HPRS-16) on passage in cell culture.马立克氏病疱疹型病毒（HPRS - 16株）在细胞培养传代过程中致瘤性丧失及毒力减弱。
J Gen Virol. 1969 Jun;4(4):557-64. doi: 10.1099/0022-1317-4-4-557.
6
Growth in agarose of human cells infected with cytomegalovirus.感染巨细胞病毒的人类细胞在琼脂糖中的生长情况。
J Virol. 1974 Aug;14(2):327-32. doi: 10.1128/JVI.14.2.327-332.1974.
7
Cytomegalovirus infections in New York State.纽约州的巨细胞病毒感染
N Y State J Med. 1974 May;74(5):785-91.
8
9
Congenital cytomegalovirus infection: consecutive occurrence due to viruses with similar antigenic compositions.先天性巨细胞病毒感染：由具有相似抗原组成的病毒连续发生。
Pediatrics. 1973 Dec;52(6):788-94.
10
Inapparent congenital cytomegalovirus infection with elevated cord IgM levels. Casual relation with auditory and mental deficiency.脐带血IgM水平升高的先天性巨细胞病毒隐性感染。与听觉和智力缺陷的因果关系。
N Engl J Med. 1974 Feb 7;290(6):291-6. doi: 10.1056/NEJM197402072900601.
Zotero 插件