Vainio Kirsti, Myrmel Mette
Department of Virology, Norwegian Institute of Public Health, Oslo, Norway.
J Clin Microbiol. 2006 Oct;44(10):3695-702. doi: 10.1128/JCM.00023-06.
During the period from January 2000 to August 2005 a total of 204 outbreaks of norovirus gastroenteritis were diagnosed at the Norwegian Institute of Public Health. A clear increase in the norovirus activity was seen in healthcare institutions during the winter seasons. Polymerase sequence analysis of norovirus strains from 122 outbreaks showed that 112 were caused by GII strains (91.8%). Two norovirus variants seen during the study period-GIIb and GII.4-were predominant between January 2000 and September 2002, whereas GII.4 was predominant from September 2002 onward. The highest norovirus activity was seen during the 2002-2003 and 2004-2005 seasons with the emergence of new GII.4 variants. This study describes the molecular epidemiology of norovirus strains circulating in Norway during the five previous seasons and compares four norovirus real-time reverse transcriptase PCR assays. A suitable assay for routine diagnostics is suggested.
在2000年1月至2005年8月期间，挪威公共卫生研究所共诊断出204起诺如病毒肠胃炎疫情。在冬季，医疗机构中诺如病毒的活跃程度明显上升。对122起疫情中的诺如病毒菌株进行的聚合酶序列分析表明，112起是由GII型菌株引起的（91.8%）。在研究期间发现的两种诺如病毒变体——GIIb和GII.4——在2000年1月至2002年9月期间占主导地位，而从2002年9月起GII.4占主导地位。在2002 - 2003年和2004 - 2005年季节出现新的GII.4变体时诺如病毒的活跃程度最高。本研究描述了前五个季节在挪威流行的诺如病毒菌株的分子流行病学，并比较了四种诺如病毒实时逆转录聚合酶链反应检测方法。建议采用一种适合常规诊断的检测方法。
