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三种市售丙型肝炎病毒基因分型方法的比较评估

Comparative evaluation of three commercially available methodologies for hepatitis C virus genotyping.

作者信息

Schutzbank Ted E, Sefers Susan E, Kahmann Nicole, Li Haijing, Tang Yi-Wei

机构信息

CompuNet Clinical Laboratories, 2308 Sandridge Rd., Dayton, OH 45439, USA.

出版信息

J Clin Microbiol. 2006 Oct;44(10):3797-8. doi: 10.1128/JCM.01159-06.

DOI:10.1128/JCM.01159-06
PMID:17021114
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1594808/
Abstract

We compared the performances of three hepatitis C virus genotyping methodologies supplied by Bayer, Abbott, and Third Wave Technologies. Genotypes were determined for 136 of 137 specimens by the Bayer method, 121 of 137 specimens by the Invader assay, and only 77 of 137 specimens by the Abbott assay. All reported genotypes were concordant by all three methods.

摘要

我们比较了拜耳公司、雅培公司和第三波技术公司提供的三种丙型肝炎病毒基因分型方法的性能。通过拜耳方法对137个样本中的136个进行了基因分型，通过侵入检测法对137个样本中的121个进行了基因分型，而通过雅培检测法仅对137个样本中的77个进行了基因分型。所有三种方法报告的基因型均一致。

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本文引用的文献

1
Evaluation of the invader assay for genotyping hepatitis C virus.丙型肝炎病毒基因分型的侵入检测法评估
J Clin Microbiol. 2006 Feb;44(2):318-23. doi: 10.1128/JCM.44.2.318-323.2006.
2
Primer sequence modification enhances hepatitis C virus genotype coverage.引物序列修饰可提高丙型肝炎病毒基因型覆盖率。
J Clin Microbiol. 2005 Jul;43(7):3576-7. doi: 10.1128/JCM.43.7.3576-3577.2005.
3
Detection of hepatitis C virus by a user-developed reverse transcriptase-PCR and use of amplification products for subsequent genotyping.用户自行研发的逆转录聚合酶链反应检测丙型肝炎病毒及使用扩增产物进行后续基因分型
J Clin Virol. 2004 Oct;31(2):148-52. doi: 10.1016/j.jcv.2004.02.010.
4
Evaluation of the TRUGENE HCV 5'NC genotyping kit with the new GeneLibrarian module 3.1.2 for genotyping of hepatitis C virus from clinical specimens.使用新型GeneLibrarian模块3.1.2的TRUGENE HCV 5'NC基因分型试剂盒对临床标本中的丙型肝炎病毒进行基因分型的评估。
J Clin Microbiol. 2003 Oct;41(10):4855-7. doi: 10.1128/JCM.41.10.4855-4857.2003.
5
Clinical evaluation of two methods for genotyping hepatitis C virus based on analysis of the 5' noncoding region.基于5'非编码区分析的两种丙型肝炎病毒基因分型方法的临床评估
J Clin Microbiol. 2003 Apr;41(4):1558-64. doi: 10.1128/JCM.41.4.1558-1564.2003.
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Laboratory assays for diagnosis and management of hepatitis C virus infection.用于丙型肝炎病毒感染诊断和管理的实验室检测
J Clin Microbiol. 2002 Dec;40(12):4407-12. doi: 10.1128/JCM.40.12.4407-4412.2002.
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First phase viral kinetic parameters as predictors of treatment response and their influence on the second phase viral decline.作为治疗反应预测指标的第一阶段病毒动力学参数及其对第二阶段病毒下降的影响。
J Viral Hepat. 2002 Sep;9(5):340-5. doi: 10.1046/j.1365-2893.2002.00377.x.
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Gastroenterology. 2001 May;120(6):1438-47. doi: 10.1053/gast.2001.24006.
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Hepatitis C virus kinetics.
Antivir Ther. 2000 Jun;5(2):85-90.
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Second-generation line probe assay for hepatitis C virus genotyping.第二代丙型肝炎病毒基因分型线探针检测法
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