Dalby Andrew B, Frank Daniel N, St Amand Allison L, Bendele Alison M, Pace Norman R
Department of Molecular, Cellular, and Developmental Biology, University of Colorado, Boulder, CO 80309-0347, USA.
Appl Environ Microbiol. 2006 Oct;72(10):6707-15. doi: 10.1128/AEM.00378-06.
Nonsteroidal anti-inflammatory drugs (NSAIDs) are commonly prescribed for a variety of inflammatory conditions; however, the benefits of this class of drugs are accompanied by deleterious side effects, most commonly gastric irritation and ulceration. NSAID-induced ulceration is thought to be exacerbated by intestinal microbiota, but previous studies have not identified specific microbes that contribute to these adverse effects. In this study, we conducted a culture-independent analysis of approximately 1,400 bacterial small-subunit rRNA genes associated with the small intestines and mesenteric lymph nodes of rats treated with the NSAID indomethacin. This is the first molecular analysis of the microbiota of the rat small intestine. A comparison of clone libraries and species-specific quantitative PCR results from rats treated with indomethacin and untreated rats revealed that organisms closely related to Enterococcus faecalis were heavily enriched in the small intestine and mesenteric lymph nodes of the treated rats. These data suggest that treatment of NSAID-induced ulceration may be facilitated by addressing the microbiological imbalances.
非甾体抗炎药（NSAIDs）常用于治疗各种炎症性疾病；然而，这类药物在带来益处的同时也伴有有害的副作用，最常见的是胃部刺激和溃疡。NSAID 诱导的溃疡被认为会因肠道微生物群而加剧，但先前的研究尚未确定导致这些不良反应的具体微生物。在本研究中，我们对约 1400 个与用 NSAID 消炎痛治疗的大鼠小肠和肠系膜淋巴结相关的细菌小亚基 rRNA 基因进行了非培养分析。这是对大鼠小肠微生物群的首次分子分析。对消炎痛治疗组大鼠和未治疗大鼠的克隆文库及物种特异性定量 PCR 结果进行比较后发现，与粪肠球菌密切相关的微生物在治疗组大鼠的小肠和肠系膜淋巴结中大量富集。这些数据表明，解决微生物失衡问题可能有助于治疗 NSAID 诱导的溃疡。
