Gianazza E, Crawford J, Miller I
Gruppo di Studio per la Proteomica e la Struttura delle Proteine, Dipartimento di Scienze Farmacologiche, Università degli Studi di Milano, Milano, Italy.
Amino Acids. 2007 Jul;33(1):51-6. doi: 10.1007/s00726-006-0410-2. Epub 2006 Oct 6.
Oxidative stress induces various post-translational modifications (PTM); some are reversible in vivo via enzymatic catalysis. The present paper reviews specific procedures for the detection of oxidative PTM in proteins, most of them including electrophoresis. Main topics are carbonylated and glutathionylated proteins as well as modification of selected amino acids (Cys, Tyr, Met, Trp, Lys).
氧化应激会诱导各种翻译后修饰（PTM）；其中一些在体内可通过酶催化作用逆转。本文综述了检测蛋白质氧化PTM的具体方法，其中大多数方法都涉及电泳。主要主题包括蛋白质羰基化和谷胱甘肽化，以及特定氨基酸（半胱氨酸、酪氨酸、甲硫氨酸、色氨酸、赖氨酸）的修饰。
